文献类型： 外文期刊

作者： Yao, Xie-Feng ¹ ; Zhang, Jiu-ming ³ ; Tian, Li ³ ; Guo, Jian-hua ² ;

作者机构： 1.Jiangsu Acad Agr Sci, Jiangsu Key Lab Hort Crop Genet Improvement, Inst Vegetable Crops, Nanjing, Jiangsu, Peoples R China

2.Nanjing Agr Univ, Dept Plant Pathol, Coll Plant Protect, Nanjing, Jiangsu, Peoples R China

3.State Ocean Adm, Inst Oceanog 1, Qingdao, Peoples R China

4.Qingdao Univ Sci & Technol, Qingdao, Peoples R China

关键词： Heavy metal contamination;PCR-DGGE fingerprinting;Multivariate analysis;Bacterial community structure

期刊名称：BRAZILIAN JOURNAL OF MICROBIOLOGY （ 影响因子：2.476； 五年影响因子：3.391 ）

ISSN： 1517-8382

年卷期： 2017 年 48 卷 1 期

页码：

收录情况： SCI

摘要： In this study, determination of heavy metal parameters and microbiological characterization of marine sediments obtained from two heavily polluted sites and one low-grade contaminated reference station at Jiaozhou Bay in China were carried out. The microbial communities found in the sampled marine sediments were studied using PCR-DGGE (denaturing gradient gel electrophoresis) fingerprinting profiles in combination with multivariate analysis. Clustering analysis of DGGE and matrix of heavy metals displayed similar occurrence patterns. On this basis, 17 samples were classified into two clusters depending on the presence or absence of the high level contamination. Moreover, the cluster of highly contaminated samples was further classified into two sub-groups based on the stations of their origin. These results showed that the composition of the bacterial community is strongly influenced by heavy metal variables present in the sediments found in the Jiaozhou Bay. This study also suggested that metagenomic techniques such as PCR-DGGE fingerprinting in combination with multivariate analysis is an efficient method to examine the effect of metal contamination on the bacterial community structure. (C) 2016 Sociedade Brasileira de Microbiologia. Published by Elsevier Editora Ltda.