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Role of Chinese herbal medicinal ingredients in secretion of cytokines by PCV2-induced endothelial cells

文献类型： 外文期刊

作者： Hu, Yiyi 1 ; He, Kongwang 2 ; Wang, Xiaomin 2 ;

作者机构： 1.Jiangsu Acad Agr Sci, Key Lab Vet Biol Engn & Technol, Natl Ctr Engn Res Vet Bioprod, Inst Vet Med,Minist Agr, Nanjing 210014, Jiangsu, Peoples R China

2.Jiangsu Acad Agr Sci, Key Lab Vet Biol Engn & Technol, Natl Ctr Engn Res Vet Bioprod, Inst Vet Med,Minist Agr, Nanjing 210014, Jiangsu, Peoples R China; Jiangsu Coinnovat Ctr Prevent & Control Important, Yangzhou, Jiangsu, Peoples R China

关键词： IL-2;IL-4;IL-10;IFN gamma;PCV2;saponins

期刊名称：JOURNAL OF IMMUNOTOXICOLOGY （ 影响因子：3.0； 五年影响因子：2.597 ）

ISSN： 1547-691X

年卷期： 2016 年 13 卷 2 期

页码：

收录情况： SCI

摘要： While T-lymphocytes are the major cell type responsible for host responses to a virus (including induction of inflammatory responses to aid in ultimate removal of virus), other cells, including macrophages, epithelial and dendritic cells also have key roles. Endothelial cells also play important roles in physiologic/pathologic processes, like inflammation, during viral infections. As endothelial cells can be activated to release various endogenous compounds, including some cytokines, ex vivo measures of cytokine formation by the cells can be used to indirectly assess any potential endothelial dysfunction in situ. The research presented here sought to investigate potential immunolomodulatory effects of five saponins on endothelial cells: Saikosaponins A (SSA) and D (SSD), Panax Notoginseng Saponin (PNS) and Notoginsenoside R1 (SR1) and Anemoside B4 (AB4). For this, cells (porcine iliac artery endothelial line) were challenged with a virus isolate PCV2-AH for 24h and then treated with the test saponin (at 1, 5 or 10g/ml) for an additional 24h at 37 degrees C. The culture supernatants were then collected and analyzed for interleukin (IL)-2, -4 and -10, as well as interferon (IFN)- by ELISA. The results revealed that PNS and SR1 inhibited the production of IL-4; PNS, SR1 and AB4 inhibited the secretion of IL-10; SSA, SSD and PNS up-regulated IL-2 expression; SSA and SSD increased the level of IFN. All these changes were significant. Taken together, the data suggested these saponins might potentially have a capacity to regulate immune responses in vivo via changes in production of these select cytokines by infected endothelial cells. Nevertheless, the impact of these agents on other key cell types involved in anti-viral responses, including T-lymphocytes, remains to be determined.

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