Flavonoid content and expression analysis of flavonoid biosynthetic genes in herbaceous peony (Paeonia lactiflora Pall.) with double colors
文献类型： 外文期刊
作者： Wu Yan-qing 1 ; Wei Meng-ran 1 ; Zhao Da-qiu 1 ; Tao Jun 1 ;
作者机构： 1.Yangzhou Univ, Jiangsu Key Lab Crop Genet & Physiol, Coll Hort & Plant Protect, Yangzhou 225009, Jiangsu, Peoples R China
2.Jiangsu Key Lab Hort Crop Genet Improvement, Nanjing 210014, Jiangsu, Peoples R China
关键词： herbaceous peony;flower color;flavonoids;gene expression
期刊名称：JOURNAL OF INTEGRATIVE AGRICULTURE （ 影响因子：2.848； 五年影响因子：2.979 ）
ISSN： 2095-3119
年卷期： 2016 年 15 卷 9 期
页码：
收录情况： SCI
摘要： Herbaceous peony (Paeonia lactiflora Pall.) is a famous flower with medicinal values, and its flowers have a number of medicinal constituents, especially flavonoids. In this study, a P. lactiflora cultivar with double colors including white outer-petal and yellow inner-petal was used as the experimental materials to perform the qualitative and quantitative analysis of flavonoids by high-performance liquid chromatograph-electrospray ionization-mass spectrometry (HPLC-ESI-MSn) and investigate the expression patterns of flavonoid biosynthetic genes using real-time quantitative polymerase chain reaction (Q-PCR). The results showed that the colors of both petals gradually weakened with flower development. Moreover, one main anthocyanin composition (peonidin 3,5-di-O-glucoside) and five main anthoxanthin compositions (kaempferol di-hexo-side, kaempferol-3-O-malonylglucoside-7-O-glucoside, quercetin-3-O-galactoside, luteolin-7-O-glucoside and isorhamnetin-3-O-glucoside) were found in the both, differing significantly in their peak areas only. Total anthocyanin, anthoxanthin and flavonoid contents in white outer-petal and yellow inner-petal gradually decreased during flower development, and were consistently higher in white outer-petal. Furthermore, the expression patterns of nine structural genes in P. lactiflora flavonoid biosynthetic pathway showed that the expression levels of phenylalanine ammonialyase gene (PIPAL), chalcone synthase gene (PICHS), flavanone 3-hydroxylase gene (PIF3H), anthocyanidin synthase gene (PIANS) and UDP-glucoside: flavonoid 5-O-glucosyltransferase gene (PIF5GT) in two petals basically presented declined tendencies, and transcription levels of PIPAL, PICHS, PIANS, PIF3GT and PIF5GT also tended to be higher in white outer-petal, which was correlated with their flavonoid contents. These results would lay a solid foundation for the exploration and utilization of flavonoid resources in P. lactiflora flowers.
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