Transcriptomics reveals the mechanism of selenium-enriched Lactobacillus plantarum alleviating brain oxidative stress under cadmium stress in Luciobarbus capito
文献类型： 外文期刊
作者： Shang, Xinchi 1 ; Geng, Longwu 1 ; Zhao, Zhigang 1 ; Luo, Liang 1 ; Shi, Xiaodan 1 ; Zhang, Qing 1 ; Du, Rujun 4 ; Cong, Yanfeng 4 ; Xu, Wei 1 ;
作者机构： 1.Chinese Acad Fishery Sci, Heilongjiang River Fisheries Res Inst, Rd 43 Songfa, Harbin 150070, Peoples R China
2.Key Lab Cold Water Fish Germplasm Resources & Mult, Harbin 150070, Heilongjiang, Peoples R China
3.Natl & Local Joint Engn Lab Freshwater Fish Breedi, Harbin, Peoples R China
4.Fisheries Technol Extens Stn Heilongjiang Prov, Daqing 166299, Peoples R China
关键词： Transcriptome analysis; Selenium-enriched Lactobacillus plantarum; Cadmium; Brain; Signaling pathway
期刊名称：ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY （ 影响因子：7.129； 五年影响因子：7.284 ）
ISSN： 0147-6513
年卷期： 2022 年 242 卷
页码：
收录情况： SCI
摘要： Cadmium (Cd) is one of toxic metal in environment and is thought to affect nervous system. There were an increasing number of studies on selenium (Se)-enriched probiotics which were believed to produce bioactive nanoselenium. The antagonism of Se on heavy metals can significantly affect biological toxicity of heavy metals. This study aimed to elucidate possible mechanism of brain injury in Luciobarbus capito after Cd exposure and the mitigation of Se-enriched probiotics through transcriptome analysis. The results revealed 465 differentially expressed genes in the Cd and the control brains (Cd vs C), including 320 genes with upregulated expression and 145 genes with downregulated expression. In addition, we found that there were 4117 differentially expressed genes in the Se-enriched L. plantarum plus Cd and the control brains (S1L1-Cd vs C), including 2552 genes with upregulated expression and 1565 genes with downregulated expression. There were 147 differentially expressed genes in the Se-enriched L. plantarum plus Cd and the control brains (S1L1-Cd vs Cd), including 40 genes with upregulated expression and 107 genes with downregulated expression. Moreover, GO enrichment analysis indicated that the differentially expressed genes were involved in biological processes cellular component, and molecular function. KEGG enrichment analysis indicated that MAPK signaling pathway, calcium signaling pathway, and PI3K-Akt signaling pathway were significantly enriched. Subsequently, qRT-PCR was performed, and we selected 15 related differentially expressed genes for verification. The qRT-PCR results revealed the same trend as the RNA-Seq results. In conclusion, this study elucidated relieving effect of Se-enriched probiotics on Cd exposure-induced brain oxidative stress. This study provided a theoretical basis for further research on genes related to Cd poisoning and the amelioration of Se-enriched probiotics on Cd poisoning.
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