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TMV mutants with poly(A) tracts of different lengths demonstrate structural variations in 3 ' UTR affecting viral RNAs accumulation and symptom expression

文献类型： 外文期刊

作者： Guo, Song 1 ; Kierzek, Elzbieta 2 ; Chen, Gang 3 ; Zhou, Yi-Jun 4 ; Wong, Sek-Man 1 ;

作者机构： 1.Natl Univ Singapore, Dept Biol Sci, Singapore 117548, Singapore

2.Polish Acad Sci, Inst Bioorgan Chem, PL-61704 Poznan, Poland

3.Nanyang Technol Univ, Sch Phys & Math Sci, Div Chem & Biol Chem, Singapore 637371, Singapore

4.Jiangsu Acad Agr Sci, Jiangsu Tech Serv Ctr Diag & Detect Plant Virus D, Inst Plant Protect, Nanjing 210014, Jiangsu, Peoples R China

5.Temasek Life Sci Lab, Singapore, Singapore

6.Natl Univ Singapore Res Inst, Suzhou, Jiangsu, Peoples R China

期刊名称：SCIENTIFIC REPORTS （ 影响因子：4.379； 五年影响因子：5.133 ）

ISSN： 2045-2322

年卷期： 2015 年 5 卷

页码：

收录情况： SCI

摘要： The upstream pseudoknots domain (UPD) of Tobacco mosaic virus (TMV) is located at the 3'-untranslated region (UTR). It plays an important role in virus replication and translation. To determine the importance of UPD and 3'-UTR, and the effects of introduced RNA elements in TMV 3'-UTR, a series of TMV mutants with internal poly(A) tract upstream of UPD was constructed for structural analysis by selective 2'-hydroxyl acylation analyzed by primer extension (SHAPE). TMV(24A+UPD) and TMV(42A+UPD) formed a similar structure as that of TMV 3'-UTR, but TMV(62A+UPD) structures altered by the introduced poly(A) tract. In addition, TMV(24A+UPD) had a higher viral RNAs accumulation than TMV in N. benthamiana protoplasts, and induced lethal symptoms in the infected plants. TMV(62A+UPD) showed a drastically reduced accumulation, its coat protein was undetectable in protoplasts, and the inoculated plants remained symptomless. This study analyzed the structures of 3'-UTR of TMV and found that the longer poly(A) tract introduced upstream of UPD reduced viral RNAs accumulation and induced milder symptoms in N. benthamiana. In conclusion, different lengths of the internal poly(A) tract introduced into the TMV 3'UTR lead to structural variations that affect virus accumulation and symptom expression.

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