Genome-wide analysis of tomato long non-coding RNAs and identification as endogenous target mimic for microRNA in response to TYLCV infection
文献类型: 外文期刊
作者: Wang, Jinyan 1 ; Yu, Wengui 1 ; Yang, Yuwen 1 ; Li, Xiao 3 ; Chen, Tianzi 1 ; Liu, Tingli 1 ; Ma, Na 1 ; Yang, Xu; Liu, R 1 ;
作者机构: 1.Jiangsu Acad Agr Sci, Prov Key Lab Agrobiol, Jiangsu Key Lab Bioresources Saline Soils, Nanjing 210014, Jiangsu, Peoples R China
2.Chinese Acad Sci, Shanghai Inst Biol Sci, Shanghai Ctr Plant Stress Biol, Shanghai 201602, Peoples R China
3.Yangzh
期刊名称:SCIENTIFIC REPORTS ( 影响因子:4.379; 五年影响因子:5.133 )
ISSN: 2045-2322
年卷期: 2015 年 5 卷
页码:
收录情况: SCI
摘要: Recently, a large number of long noncoding RNAs (lncRNAs) have emerged as important regulators of many biological processes in animals and plants. However, how lncRNAs function during plant DNA virus infection is largely unknown. We performed strand-specific paired-end RNA sequencing of tomato samples infected with Tomato yellow leaf curl virus (TYLCV) with three biological replicates. Overall, we predicted 1565 lncRNAs including long intergenic ncRNAs (lincRNAs) and natural antisense transcripts (lncNATs) and definitively identified lnRNAs that are involved in TYLCV infection by virus-induced gene silencing (VIGS). We also verified the functions of a set of lncRNAs that were differentially expressed between 0 and 7 days post inoculation (dpi). More importantly, we found that several lncRNAs acted as competing endogenous target mimics (eTMs) for tomato microRNAs involved in the TYLCV infection. These results provide new insight into lncRNAs involved in the response to TYLCV infection that are important components of the TYLCV network in tomatoes.
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