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Ethylene Regulates Levels of Ethylene Receptor/CTR1 Signaling Complexes in Arabidopsis thaliana

文献类型: 外文期刊

作者: Shakeel, Samina N. 1 ; Gao, Zhiyong 1 ; Amir, Madiha 1 ; Chen, Yi-Feng 1 ; Rai, Muneeza Iqbal 1 ; Ul Haq, Noor 1 ; Sch 1 ;

作者机构: 1.Dartmouth Coll, Dept Biol Sci, Hanover, NH 03755 USA

2.Quaid I Azam Univ, Dept Biochem, Islamabad 45320, Pakistan

3.Jiangsu Acad Agr Sci, Inst Biotechnol, Nanjing 210014, Peoples R China

关键词: Arabidopsis;histidine kinase;hormone receptor;membrane protein;protein complex;protein turnover;serine;threonine protein kinase;ethylene

期刊名称:JOURNAL OF BIOLOGICAL CHEMISTRY ( 影响因子:5.157; 五年影响因子:5.041 )

ISSN: 0021-9258

年卷期: 2015 年 290 卷 19 期

页码:

收录情况: SCI

摘要: Background: Plant responses to the hormone ethylene depend on ethylene receptors and the kinase CTR1. Results: The receptors and CTR1 exist as signaling complexes whose levels change in response to ethylene. Conclusion: A model incorporating transcriptional induction and ethylene-dependent turnover of receptor/CTR1 complexes is proposed. Significance: Results presented here reconcile molecular responses at the receptor level with physiological changes in sensitivity to ethylene. The plant hormone ethylene is perceived by a five-member family of receptors in Arabidopsis thaliana. The receptors function in conjunction with the Raf-like kinase CTR1 to negatively regulate ethylene signal transduction. CTR1 interacts with multiple members of the receptor family based on co-purification analysis, interacting more strongly with receptors containing a receiver domain. Levels of membrane-associated CTR1 vary in response to ethylene, doing so in a post-transcriptional manner that correlates with ethylene-mediated changes in levels of the ethylene receptors ERS1, ERS2, EIN4, and ETR2. Interactions between CTR1 and the receptor ETR1 protect ETR1 from ethylene-induced turnover. Kinetic and dose-response analyses support a model in which two opposing factors control levels of the ethylene receptor/CTR1 complexes. Ethylene stimulates the production of new complexes largely through transcriptional induction of the receptors. However, ethylene also induces turnover of receptors, such that levels of ethylene receptor/CTR1 complexes decrease at higher ethylene concentrations. Implications of this model for ethylene signaling are discussed.

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