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Characterization of mating-type loci in rice false smut fungus Villosiclava virens

文献类型： 外文期刊

作者： Yu, Jun-jie 1 ; Sun, Wen-xian 2 ; Yu, Mi-na 1 ; Yin, Xiao-le 1 ; Meng, Xiang-kun 1 ; Zhao, Jie 1 ; Huang, Lei 1 ; Huang, 1 ;

作者机构： 1.Jiangsu Acad Agr Sci, Inst Plant Protect, Nanjing 210014, Jiangsu, Peoples R China

2.China Agr Univ, Coll Agr & Biotechnol, Beijing 100193, Peoples R China

3.Montana State Univ, Dept Plant Sci & Plant Pathol, Bozeman, MT 59717 USA

关键词： Villosiclava virens;mating-type locus;mating-type gene;alpha-box;HMG domain

期刊名称：FEMS MICROBIOLOGY LETTERS （ 影响因子：2.742； 五年影响因子：2.856 ）

ISSN： 0378-1097

年卷期： 2015 年 362 卷 9 期

页码：

收录情况： SCI

摘要： Ascospores of Villosiclava virens are a primary infection source of rice false smut. This phytopathogenic fungus exists in heterothallic form, and mating compatibility is regulated by mating-type locus 1 (MAT1). However, the MAT1 locus structure remains unknown. The MAT1-1 and MAT1-2 idiomorphs of V. virens were characterized and annotated on the basis of cDNA sequencing. A multiplex polymerase chain reaction (PCR) method was developed to identify the mating types of hyphae and sclerotia. The MAT1-1 locus of V. virens contains three mating-type genes: MAT1-1-1, MAT1-1-2 and MAT1-1-3, and a pseudogene similar to MAT1-2-1. The MAT1-2 locus harbors the MAT1-2-1 gene and a new mating-type gene MAT1-2-8. The mRNA of MAT1-1-1, MAT1-1-3 and MAT1-2-1, but not MAT1-1-2, was detectible by reverse transcription PCR in vegetative mycelia. However, the mRNA of MAT1-1-2 was detectible in the stroma, which is a sexual reproduction structure of V. virens. A multiplex PCR detection method was developed for the identification of the MAT1-1 and MAT1-2 idiomorphs. All 20 wild-type strains harbored either the MAT1-1 or MAT1-2 idiomorphs. Sclerotia that harbored both the MAT1-1 and MAT1-2 idiomorphs had potential to form fertile stromata, whereas those that harbored only the MAT1-1 idiomorph could not form mature stromata.

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