Isolation of an aryloxyphenoxy propanoate (AOPP) herbicide-degrading strain Rhodococcus ruber JPL-2 and the cloning of a novel carboxylesterase gene (feh)
文献类型： 外文期刊
作者： Liu Hongming 1 ; Lou Xu 1 ; Ge Zhaojian; Yang Fan 1 ; Chen Dingbin 1 ; Zhu Jianchun 1 ; Xu Jianhong 1 ; Li Shunpeng 1 ; H 1 ;
作者机构： 1.Nanjing Agr Univ, Coll Life Sci, Key Lab Agr Environm Microbiol, Minist Agr, Nanjing 210095, Jiangsu, Peoples R China
2.Jiangsu Acad Agr Sci, Key Lab Agroprod Safety Risk Evaluat, Jiangsu Ctr
关键词： fenoxaprop-P-ethyl;microbial degradation;Rhodococcus ruber JPL-2;FE-hydrolyzing carboxylesterase gene
期刊名称：BRAZILIAN JOURNAL OF MICROBIOLOGY （ 影响因子：2.476； 五年影响因子：3.391 ）
ISSN： 1517-8382
年卷期： 2015 年 46 卷 2 期
页码：
收录情况： SCI
摘要： The strain JPL-2, capable of degrading fenoxaprop-P-ethyl (FE), was isolated from the soil of a wheat field and identified as Rhodococcus ruber. This strain could utilize FE as its sole carbon source and degrade 94.6% of 100 mg L-1 FE in 54 h. Strain JPL-2 could also degrade other aryloxyphenoxy propanoate (AOPP) herbicides. The initial step of the degradation pathway is to hydrolyze the carboxylic acid ester bond. A novel esterase gene feh, encoding the FE-hydrolyzing carboxylesterase (FeH) responsible for this initial step, was cloned from strain JPL-2. Its molecular mass was approximately 39 kDa, and the catalytic efficiency of FeH followed the order of FE > quizalofop-P-ethyl > clodinafop-propargyl > cyhalofop-butyl > fluazifop-P-butyl > haloxyfop-P-methyl > diclofopmethy, which indicated that the chain length of the alcohol moiety strongly affected the hydrolysis activity of the FeH toward AOPP herbicides.
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