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HETEROLOGOUS EXPRESSION OF AN ALLIGATORWEED HIGH-AFFINITY POTASSIUM TRANSPORTER GENE ENHANCES SALINITY TOLERANCE IN ARABIDOPSIS THALIANA

文献类型： 外文期刊

作者： Song, Zhizhong 1 ; Yang, Shunying 1 ; Zhu, Hong 4 ; Jin, Man 1 ; Su, Yanhua 1 ;

作者机构： 1.Chinese Acad Sci, Inst Soil Sci, State Key Lab Soil & Sustainable Agr, Nanjing 210014, Peoples R China

2.Jiangsu Acad Agr Sci, Inst Hort, Nanjing 210008, Peoples R China

3.Univ Chinese Acad Sci, Beijing 100049, Peoples R China

4.Inst Bot, Nanjing 210018, Jiangsu, Peoples R China

5.Chinese Acad Sci, Nanjing 210018, Peoples R China

关键词： alligatorweed;Alternanthera philoxeroides;Amaranthaceae;Arabidopsis thaliana;Brassicaceae;potassium;potassium homeostasis;ROS;transgenic seedlings;salinity tolerance

期刊名称：AMERICAN JOURNAL OF BOTANY （ 影响因子：3.844； 五年影响因子：3.657 ）

ISSN： 0002-9122

年卷期： 2014 年 101 卷 5 期

页码：

收录情况： SCI

摘要： Premise of the study: The potassium cation (K+), one of the most abundant cations in cells, improves plant tolerance to various abiotic stresses. Alligatorweed (Alternanthera philoxeroides) is well known for its strong capacity to accumulate K+. The distinctive K+ accumulation capability of alligatorweed is linked to a high-affinity K+ transport facilitated by K+-uptake transporters (ApKUPs). Methods: A putative K+ transporter gene, ApKUP4, was isolated from alligatorweed using degenerate primers and rapid amplification of cDNA ends (RACE) techniques. Gene expression profiles were performed by quantitative real time PCR and northern blot analysis. Moreover, we introduced ApKUP4 into Arabidopsis to determine its function in improving crop nutrition and NaCl stress tolerance. Key results: ApKUP4 was localized throughout the entire alligatorweed plant, and its expression was stimulated in the stems and roots under K+ deficiency, osmotic stress, and salinity stress. Northern blot analysis revealed that ApKUP4 was present in all tested organs of transgenic Arabidopsis plants. Compared with the wild type, Arabidopsis plants overexpressing ApKUP4 showed improved growth and K+ homeostasis. Moreover, ApKUP4 overexpression in Arabidopsis plants enhanced plant tolerance to salinity stress, as evidenced by reduced water loss and ROS generation, associated with enhanced photosynthesis, nutritional status, and enzymatic antioxidants. Conclusions: The present study provides direct evidence that the alligatorweed K+ transporter gene, ApKUP4, contributes to salinity tolerance in transgenic Arabidopsis seedlings, demonstrating the essentiality of potassium homeostasis for plant salinity tolerance.

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