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Immunization with H7-HCP-Tir-Intimin Significantly Reduces Colonization and Shedding of Escherichia coli O157:H7 in Goats

文献类型： 外文期刊

作者： Zhang, Xuehan 1 ; Yu, Zhengyu 1 ; Zhang, Shuping 5 ; He, Kongwang 1 ;

作者机构： 1.Jiangsu Acad Agr Sci, Inst Vet Med, Nanjing, Jiangsu, Peoples R China

2.Minist Agr, Key Lab Engn Res Vet Bioprod, Nanjing, Jiangsu, Peoples R China

3.Natl Ctr Engn Res Vet Bioprod, Nanjing, Jiangsu, Peoples R China

4.Jiangsu Coinnovat Ctr Prevent & Control Importan, Yangzhou, Peoples R China

5.Univ Missouri, Coll Vet Med, Dept Vet Pathobiol, Columbia, MO USA

期刊名称：PLOS ONE （ 影响因子：3.24； 五年影响因子：3.788 ）

ISSN： 1932-6203

年卷期： 2014 年 9 卷 3 期

页码：

收录情况： SCI

摘要： Enterohemorrhagic Escherichia coli (EHEC) O157:H7 is the causative agent of hemorrhagic colitis and hemolytic uremic syndrome in humans. However, the bacterium can colonize the intestines of ruminants without causing clinical signs. EHEC O157:H7 needs flagella (H7) and hemorrhagic coli pili (HCP) to adhere to epithelial cells. Then the bacterium uses the translocated intimin receptor (Tir) and an outer membrane adhesion (Intimin) protein to colonize hosts. This leads to the attachment and effacement of (A/E) lesions. A tetravalent recombinant vaccine (H7-HCP-Tir-Intimin) composed of immunologically important portions of H7, HCP, Tir and Intimin proteins was constructed and its efficacy was evaluated using a caprine model. The results showed that the recombinant vaccine induced strong humoral and mucosal immune responses and protected the subjects from live challenges with EHEC O157:H7 86-24 stain. After a second immunization, the average IgG titer peaked at 7.2x10(5). Five days after challenge, E. coli O157:H7 was no longer detectable in the feces of vaccinated goats, but naive goats shed the bacterium throughout the course of the challenge. Cultures of intestinal tissues showed that vaccination of goats with H7-HCP-Tir-Intimin reduced the amount of intestinal colonization by EHEC O157:H7 effectively. Recombinant H7-HCP-Tir-Intimin protein is an excellent vaccine candidate. Data from the present study warrant further efficacy studies aimed at reducing EHEC O157:H7 load on farms and the contamination of carcasses by this zoonotic pathogen.

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