Conserved miRNAs and Their Response to Salt Stress in Wild Eggplant Solanum linnaeanum Roots
文献类型: 外文期刊
作者: Zhuang, Yong 1 ; Zhou, Xiao-Hui 1 ; Liu, Jun 1 ;
作者机构: 1.Jiangsu Acad Agr Sci, Inst Vegetable Crops, Nanjing 210014, Jiangsu, Peoples R China
关键词: miRNA;high-throughput sequencing;Solanum linnaeanum;salt stress
期刊名称:INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES ( 影响因子:5.923; 五年影响因子:6.132 )
ISSN: 1422-0067
年卷期: 2014 年 15 卷 1 期
页码:
收录情况: SCI
摘要: The Solanaceae family includes some important vegetable crops, and they often suffer from salinity stress. Some miRNAs have been identified to regulate gene expression in plant response to salt stress; however, little is known about the involvement of miRNAs in Solanaceae species. To identify salt-responsive miRNAs, high-throughput sequencing was used to sequence libraries constructed from roots of the salt tolerant species, Solanum linnaeanum, treated with and without NaCl. The sequencing identified 98 conserved miRNAs corresponding to 37 families, and some of these miRNAs and their expression were verified by quantitative real-time PCR. Under the salt stress, 11 of the miRNAs were down-regulated, and 3 of the miRNAs were up-regulated. Potential targets of the salt-responsive miRNAs were predicted to be involved in diverse cellular processes in plants. This investigation provides valuable information for functional characterization of miRNAs in S. linnaeanum, and would be useful for developing strategies for the genetic improvement of the Solanaceae crops.
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