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Distribution and genetic diversity of Southern rice black-streaked dwarf virus in China

文献类型: 外文期刊

作者: Cheng, Zhaobang 1 ; Li, Shuo 1 ; Gao, Ruizhen 1 ; Sun, Feng 1 ; Liu, Wancai 2 ; Zhou, Guohui 3 ; Wu, Jianxiang 4 ; Zhou, 1 ;

作者机构: 1.Jiangsu Acad Agr Sci, Inst Plant Protect, Jiangsu Tech Serv Ctr Diag & Detect Plant Virus D, Nanjing 210014, Jiangsu, Peoples R China

2.Natl Agrotech Extens & Serv Ctr, Beijing 100125, Peoples R China

3.South China Agr Univ, Coll Nat Resources & Environm, Guangzhou 510642, Guangdong, Peoples R China

4.Zhejiang Univ, State Key Lab Rice Biol, Inst Biotechnol, Hangzhou 310058, Zhejiang, Peoples R China

5.Zhejiang Univ, State Key Lab Rice Biol, Inst Biotechnol, Hangzhou 310058, Zhejiang,

关键词: Southern rice black-streaked dwarf virus;Viral distribution in China;Genetic diversity of SRBSDV

期刊名称:VIROLOGY JOURNAL ( 影响因子:4.099; 五年影响因子:3.719 )

ISSN: 1743-422X

年卷期: 2013 年 10 卷

页码:

收录情况: SCI

摘要: Background: Rice and maize dwarf diseases caused by the newly introduced Southern rice black-streaked dwarf virus (SRBSDV) have led to severe economic losses in South China in recent years. The distribution and diversity of SRBSDV have not been investigated in the main rice and maize growing areas in China. In this study, the distribution of SRBSDV in China was determined by using reverse transcription-polymerase chain reaction (RT-PCR). Results: Between 2009 and 2010, 2404 plant samples (2294 rice, 110 maize samples, and more than 300 cultivars) with dwarf symptoms were collected from fields in 194 counties of 17 provinces in China and SRBSDV was detected. The results indicated that 1545 (64.27%) of samples (both rice and maize) were infected with SRBSDV. SRBSDV was detected widely in Hainan, Guangdong, Guangxi, Yunnan, Guizhou, Chongqing, Fujian, Jiangxi, Hunan, Hubei, Anhui, Jiangsu, and Zhejiang provinces, which suggests SRBSDV is an important pathogen causing rice dwarfing diseases in South China. Phylogenetic analysis of 15 representative virus isolates revealed that SRBSDV isolates in China had high levels of nucleotide and amino acid sequence identities (>97.8%). Conclusions: SRBSDV spreads naturally in Yangtze River basin and south region, the location of the major rice production areas. In comparison, the virus rarely spreads north of Yangtze River in North China. Distribution of SRBSDV is consistent with the migrating and existing ranges of its vector WBPH, suggesting that SRBSDV might be introduced into South China along with the migration of viruliferous WBPH.

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[1]Development of SYBR Green I-Based One-Step Real Time RT-PCR Assay for Quantifying Southern rice black- streaked dwarf virus in Rice. Zhou, Tong,Du, Linlin,Lan, Ying,Sun, Feng,Fan, Yongjian,Zhou, Yijun. 2014

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