您好，欢迎访问江苏省农业科学院 机构知识库！
江苏省农业科学院机构知识库

全部

  • 全部
  • 标题
  • 学者
  • 机构
  • 关键词

Simultaneous determination of deoxynivalenol, and 15-and 3-acetyldeoxynivalenol in cereals by HPLC-UV detection

文献类型： 外文期刊

作者： Yang, D. 1 ; Geng, Z. M. 1 ; Yao, J. B. 1 ; Zhang, X. 1 ; Zhang, P. P. 1 ; Ma, H. X. 1 ;

作者机构： 1.Jiangsu Acad Agr Sci, Prov Key Lab Agrobiol, Nanjing 210014, Peoples R China

关键词： Fusarium graminearum;Fusarium head blight;deoxynivalenol;acetyldeoxynivalenol;HPLC-UV

期刊名称：WORLD MYCOTOXIN JOURNAL （ 影响因子：3.353； 五年影响因子：3.108 ）

ISSN： 1875-0710

年卷期： 2013 年 6 卷 2 期

页码：

收录情况： SCI

摘要： Fusarium head blight is an important cereal crop disease, which not only causes yield losses but also mycotoxin contamination in wheat and other cereal grains. Developing an accurate, rapid and efficient assay is critical to minimise the risk of Fusarium mycotoxins for human and animal health. In this study, HPLC with UV detection was used to separate and quantify deoxynivalenol, 15-acetyldeoxynivalenol and 3-acetyldeoxynivalenol in cereals. Samples were extracted with water, and the extracting solution was precipitated by adding an equal volume of ethanol followed by solid-phase extraction. The analytes were separated on a reversed-phase C18 column by a mobile phase composed of acetonitrile and 1 mM H3PO4 with gradient elution. 15- and 3-acetyldeoxynivalenol showed effective baseline separation. All analytes were well-resolved from matrix co-extractives and detected at 224 nm. The results showed good linearity of calibration curves (R-2 ranged from 0.997 to 0.999) and excellent precision for inter- and intra-day determinations. Average recovery rates for the tested matrices ranged from 71 to 92%. The limits of detection and quantification ranged from 16 to 25 ng/g and 48 to 60 ng/g, respectively. The results indicate that the feasibility and practicality of the presented LC-UV method are excellent and that the method is suitable for routine analysis of DON and its acetyl derivatives in cereal grains.

  • 相关文献

[1]Quantitative trait loci for resistance to fusarium head blight and deoxynivalenol accumulation in Wangshuibai wheat under field conditions. Ma, H. X.,Zhang, K. M.,Gao, L.,Bai, G. H.,Chen, H. G.,Cai, Z. X.,Lu, W. Z..

[2]Effects of plant height on type I and type II resistance to fusarium head blight in wheat. Yan, W.,Li, H. B.,Liu, C. J.,Yan, W.,Cai, S. B.,Ma, H. X.,Rebetzke, G. J.,Liu, C. J..

[3]脱氧雪腐镰刀菌烯醇(DON)降解菌Devosia sp. A8和Paracoccus yeei A9的生理生化及生长特性. 王燕霞,王刚,王宇,戴亦军,史建荣,江凌,徐剑宏. 2023

[4]Relationship of Deoxynivalenol Content in Grain, Chaff, and Straw with Fusarium Head Blight Severity in Wheat Varieties with Various Levels of Resistance. Ji, Fang,Wu, Jirong,Zhao, Hongyan,Xu, Jianhong,Shi, Jianrong,Ji, Fang,Wu, Jirong,Zhao, Hongyan,Xu, Jianhong,Shi, Jianrong,Ji, Fang,Wu, Jirong,Zhao, Hongyan,Xu, Jianhong,Shi, Jianrong,Ji, Fang,Wu, Jirong,Zhao, Hongyan,Xu, Jianhong,Shi, Jianrong. 2015

[5]The septins FaCdc3 and FaCdc12 are required for cytokinesis and affect asexual and sexual development, lipid metabolism and virulence in Fusarium asiaticum. Zhang, Yu,Gao, Tao,Shao, Wenyong,Zheng, Zhitian,Zhou, Mingguo,Chen, Changjun,Gao, Tao. 2017

[6]Natural occurrence of deoxynivalenol and zearalenone in wheat from Jiangsu province, China. Ji, Fang,Xu, Jianhong,Liu, Xin,Yin, Xianchao,Shi, Jianrong.

[7]Resistance to Fusarium head Blight and Deoxynivalenol Accumulation in Chinese Barley. Ma, Hongxiang,Ge, Hejing,Zhang, Xu,Lu, Weizhong,Yu, Dazhao,Chen, He,Chen, Jianming.

[8]Effect of preceding crop on Fusarium species and mycotoxin contamination of wheat grains. Qiu, Jianbo,Dong, Fei,Yu, Mingzheng,Xu, Jianhong,Shi, Jianrong,Qiu, Jianbo,Dong, Fei,Yu, Mingzheng,Xu, Jianhong,Shi, Jianrong,Qiu, Jianbo,Dong, Fei,Yu, Mingzheng,Xu, Jianhong,Shi, Jianrong,Qiu, Jianbo,Dong, Fei,Yu, Mingzheng,Xu, Jianhong,Shi, Jianrong.

[9]Natural occurrence of deoxynivalenol and deoxynivalenol-3-glucoside in various wheat cultivars grown in Jiangsu province, China. Dong, F.,Wang, S.,Yu, M.,Xu, J.,Shi, J.,Dong, F.,Wang, S.,Yu, M.,Xu, J.,Shi, J.,Dong, F.,Wang, S.,Yu, M.,Xu, J.,Shi, J.,Dong, F.,Wang, S.,Yu, M.,Xu, J.,Shi, J.,Dong, F.,Wang, S.,Yu, M.,Xu, J.,Shi, J.,Sun, Y..

[10]Change of Defensive-related Enzyme in Wheat Crown Rot Seedlings Infected by Fusarium graminearum. Zhang, P.,Zhou, M. P.,Zhang, X.,Huo, Y.,Ma, H. X.. 2013

[11]Enzyme-Linked Immunosorbent-Assay for Deoxynivalenol (DON). Ji, Fang,Li, Hua,Xu, Jianhong,Shi, Jianrong. 2011

[12]Fusarium graminearum growth inhibition due to glucose starvation caused by osthol. Shi, Zhiqi,Shen, Shouguo,Zhou, Wei,Wang, Fei,Fan, Yongjian. 2008

[13]Genetic Relationships, Carbendazim Sensitivity and Mycotoxin Production of the Fusarium Graminearum Populations from Maize, Wheat and Rice in Eastern China. Qiu, Jianbo,Shi, Jianrong. 2014

[14]Expression of a radish defensin in transgenic wheat confers increased resistance to Fusarium graminearum and Rhizoctonia cerealis. Li, Zhao,Zhang, Zengyan,Du, Lipu,Xu, Huijun,Xin, Zhiyong,Li, Zhao,Zhou, Miaoping,Ren, Lijuan,Zhang, Boqiao.

[15]Involvement of threonine deaminase FgIlv1 in isoleucine biosynthesis and full virulence in Fusarium graminearum. Liu, Xin,Xu, Jianhong,Wang, Jian,Ji, Fang,Yin, Xianchao,Shi, Jianrong.

[16]Hexokinase plays a critical role in deoxynivalenol (DON) production and fungal development in Fusarium graminearum. Zhang, Leigang,Li, Baicun,Zhang, Yu,Jia, Xiaojing,Zhou, Mingguo,Zhang, Leigang,Li, Baicun,Zhang, Yu,Jia, Xiaojing,Zhou, Mingguo,Zhang, Leigang.

[17]Involvement of FgMad2 and FgBub1 in regulating fungal development and carbendazim resistance in Fusarium graminearum. Zhang, L. G.,Zhang, Y.,Li, B. C.,Jia, X. J.,Chen, C. J.,Zhou, M. G.,Zhang, L. G..

[18]Molecular characterization of the Fusarium graminearum species complex in Eastern China. Qiu, Jianbo,Xu, Jianhong,Shi, Jianrong,Qiu, Jianbo,Xu, Jianhong,Shi, Jianrong,Qiu, Jianbo,Xu, Jianhong,Shi, Jianrong.

[19]The Fungicidal Activity of Thymol against Fusarium graminearum via Inducing Lipid Peroxidation and Disrupting Ergosterol Biosynthesis. Gao, Tao,Chen, Jian,Shi, Zhiqi,Gao, Tao,Chen, Jian,Shi, Zhiqi,Gao, Tao,Chen, Jian,Shi, Zhiqi,Zhou, Hao,Zhou, Wei,Hu, Liangbin.

[20]Molecular characterization of Fusarium head blight resistance from wheat variety Wangshuibai. Zhang, X,Zhou, MP,Ren, LJ,Bai, GH,Ma, HX,Scholten, OE,Guo, PG,Lu, WZ. 2004

作者其他论文 更多>>
置顶
购物车
反馈

© 京ICP备09089781号-29 主办单位：江苏省农业科学院

学术资源： 中国农业科学院 农业专业知识服务系统 农科联盟资源共建共享平台 中国农业科技文献与信息服务平台 中国农业期刊集成服务平台