Genome-Wide Sequence Characterization and Expression Analysis of Major Intrinsic Proteins in Soybean (Glycine max L.)
文献类型： 外文期刊
作者： Zhang, Da Yong 1 ; Ali, Zulfiqar 1 ; Wang, Chang Biao 3 ; Xu, Ling 1 ; Yi, Jin Xin 1 ; Xu, Zhao Long 1 ; Liu, Xiao Qin 1 ;
作者机构： 1.Jiangsu Acad Agr Sci, Inst Biotechnol, Nanjing, Jiangsu, Peoples R China
2.Univ Agr Faisalabad, Dept Plant Breeding & Genet, Faisalabad, Pakistan
3.Shanxi Agr Sci, Cotton Res Inst, Yuncheng, Peoples R China
4.Nanjing Agr Univ, Nanjing, Jiangsu, Peoples R China
5.Univ Agr Faisalabad, Coll Agr & Appl Biol, Faisalabad, Pakistan
6.Univ Sydney, Plant Breeding Inst, Sydney, NSW 2006, Australia
期刊名称：PLOS ONE （ 影响因子：3.24； 五年影响因子：3.788 ）
ISSN： 1932-6203
年卷期： 2013 年 8 卷 2 期
页码：
收录情况： SCI
摘要： Water is essential for all living organisms. Aquaporin proteins are the major facilitator of water transport activity through cell membranes of plants including soybean. These proteins are diverse in plants and belong to a large major intrinsic (MIP) protein family. In higher plants, MIPs are classified into five subfamilies including plasma membrane intrinsic proteins (PIP), tonoplast intrinsic proteins (TIP), NOD26-like intrinsic proteins (NIP), small basic intrinsic proteins (SIP), and the recently discovered X intrinsic proteins (XIP). This paper reports genome wide assembly of soybean MIPs, their functional prediction and expression analysis. Using a bioinformatic homology search, 66 GmMIPs were identified in the soybean genome. Phylogenetic analysis of amino acid sequences of GmMIPs divided the large and highly similar multi-gene family into 5 subfamilies: GmPIPs, GmTIPs, GmNIPs, GmSIPs and GmXIPs. GmPIPs consisted of 22 genes and GmTIPs 23, which showed high sequence similarity within subfamilies. GmNIPs contained 13 and GmSIPs 6 members which were diverse. In addition, we also identified a two member GmXIP, a distinct 5th subfamily. GmMIPs were further classified into twelve subgroups based on substrate selectivity filter analysis. Expression analyses were performed for a selected set of GmMIPs using semi-quantitative reverse transcription (semi-RT-qPCR) and qPCR. Our results suggested that many GmMIPs have high sequence similarity but diverse roles as evidenced by analysis of sequences and their expression. It can be speculated that GmMIPs contains true aquaporins, glyceroporins, aquaglyceroporins and mixed transport facilitators.
- 相关文献
作者其他论文 更多>>
-
GmWRKY49, a Salt-Responsive Nuclear Protein, Improved Root Length and Governed Better Salinity Tolerance in Transgenic Arabidopsis
作者：Xu, Zhaolong;Xu, Ling;He, Xiaolan;Huang, Yihong;Yi, Jinxin;Zhang, Dayong;Shao, Hong-Bo;Raza, Qasim;Ali, Zulfiqar;Raza, Qasim;Shao, Hong-Bo;Ma, Hongxiang
关键词：Glycine max; GmWRKY49; overexpression; salinity tolerance; WRKY TFs
-
The Soybean Basic Helix-Loop-Helix Transcription Factor ORG3-Like Enhances Cadmium Tolerance via Increased Iron and Reduced Cadmium Uptake and Transport from Roots to Shoots
作者：Xu, Zhaolong;Liu, Xiaoqing;He, Xiaolan;Xu, Ling;Huang, Yihong;Shao, Hongbo;Zhang, Dayong;Shao, Hongbo;Tang, Boping;Ma, Hongxiang
关键词：soybean;bHLH transcription factor;GmORG3;cadmium tolerance;gene expression
-
Genome-wide identification of Major Intrinsic Proteins in Glycine soja and characterization of GmTIP2;1 function under salt and water stress
作者：Zhang, Da-yong;Xu, Ling;Wan, Qun;Huang, Yi-hong;Xu, Zhao-Long;He, Xiao-Lan;Shao, Hong-Bo;Kumar, Manoj;Pandey, Girdhar K.;Ma, Jin-Biao;Shao, Hong-Bo;Kumar, Manoj
关键词：
-
BIOCHEMICAL EVALUATION FOR WEAK-LIGHT TOLERANCE OF VARIOUS TOMATO (SOLANUM LYCOPERSICUM L.) CULTIVARS IN CHINA
作者：Xu, Xincheng;Song, Weiyi;Ding Jinping;Shao, Hongbo;Ali, Zulfiqar;Faridi, Rabia
关键词：Tomato cultivars;Biochemical indices;Soil management;Main factor analysis;Subordinate function analysis
-
The nuclear protein GmbZIP110 has transcription activation activity and plays important roles in the response to salinity stress in soybean
作者：Xu, Zhaolong;Xu, Ling;He, Xiaolan;Huang, Yihong;Yi, Jinxin;Shao, Hongbo;Ma, Hongxiang;Zhang, Dayong;Ali, Zulfiqar
关键词：
-
A Novel Soybean Intrinsic Protein Gene, GmTIP2;3, Involved in Responding to Osmotic Stress
作者：Zhang, Dayong;He, Xiaolan;Xu, Zhaolong;Xu, Ling;Wei, Peipei;Huang, Yihong;Brestic, Marian;Ma, Hongxiang;Shao, Hongbo;Tong, Jinfeng;Brestic, Marian;Shao, Hongbo
关键词：soybean;GmTIP2;3;heterologous expression;promoter;osmotic stress
-
Soybean C2H2-Type Zinc Finger Protein GmZFP3 with Conserved QALGGH Motif Negatively Regulates Drought Responses in Transgenic Arabidopsis
作者：Zhang, Dayong;Xu, Zhaolong;Wei, Peipei;Xu, Ling;Wan, Qun;Huang, Yihong;He, Xiaolan;Shao, Hongbo;Ma, Hongxiang;Tong, Jinfeng;Yang, Jiayin;Shao, Hongbo
关键词：Soybean;GmZFP3;transgenic Arabidopsis;drought response;negatively
