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Analysis on Differentially Expressed Genes of Muscle Tissues in Rugao Chicken at the Ages of 2 and 12 Weeks by Microarray

文献类型： 外文期刊

作者： Luan, De Qin 1 ; Bin Chang, Guo 1 ; Zhen, Ting 1 ; Huang, Zheng Yang 1 ; Zhou, Wei 1 ; Liu, Yan 2 ; Chen, Guo Hong 1 ;

作者机构： 1.Yangzhou Univ, Coll Anim Sci & Technol, Yangzhou 225009, Peoples R China

2.Jiangsu Acad Agr Sci, Inst Agr Resources & Environm Res, Engn Res Ctr Digital Agr, Nanjing 210014, Jiangsu, Peoples R China

关键词： chicken;GeneChip;muscle tissues;gene expression profile;real-time quantitative PCR

期刊名称：THAI JOURNAL OF VETERINARY MEDICINE （ 影响因子：0.281； 五年影响因子：0.465 ）

ISSN： 0125-6491

年卷期： 2011 年 41 卷 3 期

页码：

收录情况： SCI

摘要： Microarray containing 13319 probes was used to construct gene expression profiles in order to screen differentially expressed genes of muscle tissues in Rugao chicken and investigate the molecular mechanism related with muscle tissue traits at 2 and 12 weeks old of chicken. Two hundred and eight differentially expressed genes, of which 108 were known genes, 94 up-regulated, 114 down-regulated were involved in growth, molecular mechanism, fat metabolism, cell proliferation, transcription and splicing factor, protein synthesis and degradation. Meanwhile, some genes of these differentially expressed genes that had no annotation in GenBank were screened out, they were presumed to be unknown new genes. The differential expressions of 4 genes were confirmed by real-time quantitative PCR and the results indicated that the expression changes of these genes were generally consistent with the results of Gene Chip, a technology that could be used in breeding and selection of Chinese native chicken breeds.

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