文献类型： 外文期刊
作者： Guo, Dingqian 1 ; Tang, Cheng 1 ; Hai, Quan 1 ; Shao, Guoqing 2 ; Yue, Hua 1 ;
作者机构： 1.SW Univ Nationalities, Coll Life Sci & Technol, Chengdu 610041, Peoples R China
2.Jiangsu Acad Agr Sci, Nanjing 210041, Peoples R China
关键词： diagnostic antisera;Haemophilus parasuis;plate-agglutination
期刊名称：JOURNAL OF VETERINARY SCIENCE （ 影响因子：1.672； 五年影响因子：1.904 ）
ISSN： 1229-845X
年卷期： 2010 年 11 卷 4 期
页码：
收录情况： SCI
摘要： Due to the serovar diversity in Haemophilus (H.) parasuis, it is difficult to develop a universal serological method for detection of this pathogen. Here, we report a universal plate-agglutination test for detecting H. parasitic. Diagnostic antisera were prepared by mixing antisera of serovars 4, 5, 12, 13 and 14 in the optimized ratio. The results of the plate-agglutination test showed that the diagnostic antisera could agglutinate with all 15 reference strains of H. parasuis and 74/75 clinical isolates. Further, the specificity of the method was validated with 22 bacterial strains from 12 related species.
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