文献类型: 外文期刊
作者: Yan Li-na 1 ; Li Xia 1 ; Wu Dan 1 ;
作者机构: 1.Jiangsu Acad Agr Sci, Jiangsu High Qual Rice R&D Ctr, Inst Food Crop, Nanjing 210014, Peoples R China
2.Nanjing Agr Univ, Coll Life Sci, Nanjing 210095, Peoples R China
关键词: japonica;indica;hybrid rice;mature embryos;hormone;tissue culture ability
期刊名称:AGRICULTURAL SCIENCES IN CHINA ( 影响因子:0.82; 五年影响因子:0.997 )
ISSN: 1671-2927
年卷期: 2010 年 9 卷 6 期
页码:
收录情况: SCI
摘要: In order to study the regeneration technology of mature embryos in different rice varieties, nine japonica, nine indica and eleven hybrid rice varieties of two line or three line or superiority combinations were selected as explants to study the callus induction, differentiation and regeneration rates on different media The higher callus induction (61 7-89 2%) was observed in japonica rice, when cytokinin was added at lower concentration (0 3 mg L(-1) 6-BA) in M8 basal medium, supplemented with 30 g L(-1) sucrose, 8 g L(-1) agar and 2 mg L(-1) 2,4-D. Further, the addition of two cytokinins (2 mg L(-1) 6-BA, 0.5 mg L(-1) KT) and 1 mg L(-1) NAA in the M8 basal supplemented medium resulted in 9 1-100% of the callus induction in indica rice. The percent callus induction in hybrid rice varieties was 40-86.3% when addition of 1 mg L(-1) 6-BA and 1 mg L-1 KT was added, and the cytokinins was required by the Japonica and indica rice varieties in the M8 basal supplemented medium It was observed that when the 0.5 mg L(-1) 2,4-D and 1 mg L(-1) 6-BA were added in japonica rice, and 0.2 mg L(-1) 2,4-D and 0.5 mg L(-1) 6-BA were added in indica and hybrid rice in the MS different media, the regeneration rates were 9.2-59 5%, 3 6-87.5% and 17 2-43 2% for Japonica, indica and hybrid rice, respectively. Thus, the regeneration technology with higher output is established in the mature embryos of similar rice varieties.
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