文献类型: 外文期刊
作者: Wang, Chunmei 1 ; Zhang, Jie; Chen, Hao 1 ; Fan, Yongjian 1 ; Shi, Zhiqi 1 ;
作者机构: 1.Jiangsu Acad Agr Sci, Food Safety Res & Serv Inst, Nanjing 210014, Peoples R China
2.State Key Lab Breeding Base, Key Lab Food Safety & Qual Jiangsu Prov, Nanjing 210014, Peoples R China
3.Minist Agr, Key Lab Food Safety & Management, Nanjing 210014, Peoples R China
4.Minist Agr, Key Lab Food Safety & Manag
关键词: natural compound;fungal diseases;mode of action;plasma membrane
期刊名称:TROPICAL PLANT PATHOLOGY ( 影响因子:1.488; 五年影响因子:1.675 )
ISSN: 1982-5676
年卷期: 2010 年 35 卷 3 期
页码:
收录情况: SCI
摘要: The antifungal properties of eugenol were tested against ten plant pathogenic fungal species and detailed studies were conducted regarding in vitro activity of eugenol on Botrytis cinerea. The EC(50) value of eugenol on mycelial radial growth of B. cinerea was 38.6 mu g/mL; however, eugenol had no bioactivity against conidia germination. B. cinerea hyphae treated with eugenol showed strong propidium iodide fluorescence in the cytosol. Eugenol increased the concentration of potassium ion and cellular materials in the medium. Furthermore, light and scanning electron microscopy observations on hyphae exposed to eugenol revealed considerable morphological alterations in hyphae, such as cytoplasmic coagulation, vacuolation, and hyphal shriveling. Eugenol induced the generation of H(2)O(2) and increased free Ca(2+) in the cytoplasm. These results strongly support the idea that the antifungal activity of eugenol is due to membrane binding and permeability alteration, leading to destabilization and disruption of the plasma membrane.
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