您好，欢迎访问江苏省农业科学院 机构知识库！
江苏省农业科学院机构知识库

全部

  • 全部
  • 标题
  • 学者
  • 机构
  • 关键词

Immune Responses to the Attenuated Mycoplasma hyopneumoniae 168 Strain Vaccine by Intrapulmonic Immunization in Piglets

文献类型： 外文期刊

作者： Feng Zhi-xin 1 ; Shao Guo-qing 1 ; Liu Mao-jun 1 ; Wu Xu-su 1 ; Zhou Yong-qi 1 ; Gan Yuan 1 ;

作者机构： 1.Jiangsu Acad Agr Sci, Minist Agr, Natl Ctr Engn Res Vet Bioprod, Key Lab Anim Dis Diagnost & Immunol,Inst Vet Med, Nanjing 210014, Peoples R China

关键词： Mycoplasma hyopneumoniae;attenuated vaccine;cellular immunity;mucosal immunity

期刊名称：AGRICULTURAL SCIENCES IN CHINA （ 影响因子：0.82； 五年影响因子：0.997 ）

ISSN： 1671-2927

年卷期： 2010 年 9 卷 3 期

页码：

收录情况： SCI

摘要： To investigate the immune responses to the attenuated Mycoplasma hyopneumoniae 168 strain vaccine, 8-15 d old piglets were immunized with M. hyopneumoniae 168 strain vaccine by intrapulmonic route. And the specific IgG antibody in serum, lymphoproliferation, IFN gamma, and specific secretory IgA (SIgA) antibody in bronchoalveolar lavage fluid were detected on 30 and 60 d post-immunization (DPI), respectively. On 60 DPI, all the pigs except for those in health control group were challenged with a field M. hyopneumoniae strain JS. Necropsy was performed on 30 d post-challenge (DPC). The results showed that IFN gamma and specific SIgA were stimulated on surface of respiratory tract after immunization. And peripheral blood mononuclear cells could also be proliferated about 1.81 and 2.12 fold on 30 and 60 DPI when stimulated by M. hyopneumoniae protein in vitro. However, no serum IgG antibody against M. hyopneumoniae was detected during the whole immune phage. After challenge, vaccinated pigs were observed with only very slight histological lesion in individual lobes. None of vaccinated pigs showed any clinical signs. While the unvaccinated pigs from challenge control group showed varying degrees of clinical sign and severe macroscopical lesion of mycoplasmal pneumonia of swine (MPS). The result suggested that the attenuated M. hyopneumoniae 168 strain vaccine inoculated by intrapulmonic route could activate the systemic cellular immunity, the local mucosal immunity and IFN gamma secretion in respiratory tract to against M. hyopneumoniae infection in piglets.

  • 相关文献

[1]Comparative analysis of mucosal immunity to Mycoplasma hyopneumoniae in Jiangquhai porcine lean strain and DLY piglets. Hua, L. Z.,Wu, Y. Z.,Bai, F. F.,William, K. K.,Feng, Z. X.,Liu, M. J.,Yao, J. T.,Zhang, X.,Shao, G. Q.. 2014

[2]Development and validation of an attenuated Mycoplasma hyopneumoniae aerosol vaccine. Feng, Zhi-Xin,Wei, Yan-Na,Li, Gui-Lan,Lu, Xiao-Ming,Wang, Zhan-Wei,Kong, Meng,Gan, Yuan,Bai, Fang-Fang,Liu, Mao-Jun,Xiong, Qi-Yan,Wu, Xu-Su,Shao, Guo-Qing,Wan, Xiu-Feng,Pharr, G. Todd. 2013

[3]A rapid and sensitive loop-mediated isothermal amplification procedure (LAMP) for Mycoplasma hyopneumoniae detection based on the p36 gene. Liu, M. J.,Du, G. M.,Bai, F. F.,Wu, Y. Z.,Xiong, Q. Y.,Feng, Z. X.,Li, B.,Shao, G. Q.,Du, G. M.,Liu, M. J.,Shao, G. Q.. 2015

[4]Cholesterol exacerbates Mycoplasma hyopneumoniae-induced apoptosis via stimulating proliferation and adhesion to porcine alveolar macrophages. Liu, Maojun,Hu, Yun,Liu, Jie,Jia, Yimin,Zhao, Ruqian,Liu, Maojun,Liu, Beibei,Shao, Guoqing,Jia, Yimin,Zhao, Ruqian,Du, Gaimei,Minion, F. Chris,Shao, Guoqing. 2017

[5]CYP1A1 mediates the suppression of major inflammatory cytokines in pulmonary alveolar macrophage (PAM) cell lines caused by Mycoplasma hyponeumoniae. Fang, Xiaomin,Zhao, Weimin,Xu, Jie,Tu, Feng,Wang, Xuemin,Li, Bixia,Fu, Yanfeng,Ren, Shouwen.

[6]Apoptosis induced by lipid-associated membrane proteins from Mycoplasma hyopneumoniae in a porcine lung epithelial cell line with the involvement of caspase 3 and the MAPK pathway. Ni, B.,Bai, F. F.,Wei, Y.,Liu, M. J.,Feng, Z. X.,Xiong, Q. Y.,Hua, L. Z.,Shao, G. Q.. 2015

[7]Development of a blocking ELISA for detection of Mycoplasma hyopneumoniae infection based on a monoclonal antibody against protein P65. Liu, Maojun,Zhang, Yue,Wu, Yuzi,Wang, Haiyan,Li, Bin,Bai, Yun,Feng, Zhixin,Xiong, Qiyan,Bai, Fangfang,Shao, Guoqing,Liu, Maojun,Du, Gaimei,Browning, Glenn F.,Shao, Guoqing.

[8]Effect of different adjuvant formulations on the immunogenicity and protective effect of a live Mycoplasma hyopneumoniae vaccine after intramuscular inoculation. Wang, Chunlai,Xie, Fang,Xiong, Qiyan,Wei, Yanna,Xie, Haidong,Feng, Zhixin,Gan, Yuan,Liu, Maojun,Bai, Fangfang,Shao, Guoqing.

[9]Comparative genomic analyses of Mycoplasma hyopneumoniae pathogenic 168 strain and its high-passaged attenuated strain. Liu, Wei,Xiao, Shaobo,Li, Mao,Guo, Shaohua,Li, Sha,Luo, Rui,Chen, Huanchun,Fang, Liurong,Feng, Zhixin,Li, Bin,Shao, Guoqing,Zhou, Zhemin. 2013

[10]Vaccination inhibits TLR2 transcription via suppression of GR nuclear translocation and binding to TLR2 promoter in porcine lung infected with Mycoplasma hyopneumoniae. Sun, Zhiyuan,Liu, Maojun,Zou, Huafeng,Li, Xian,Zhao, Ruqian,Liu, Maojun,Shao, Guoqing. 2013

[11]A replicating plasmid-based vector for GFP expression in Mycoplasma hyopneumoniae. Ishag, H. Z. A.,Liu, M. J.,Yang, R. S.,Xiong, Q. Y.,Feng, Z. X.,Shao, G. Q.,Ishag, H. Z. A.. 2016

[12]Protective efficacy of a live attenuated Mycoplasma hyopneumoniae vaccine with an ISCOM-matrix adjuvant in pigs. Xiong, Qiyan,Wei, Yanna,Feng, Zhixin,Gan, Yuan,Liu, Zhanjun,Liu, Maojun,Bai, Fangfang,Shao, Guoqing.

[13]In vitro protective efficacy of Lithium chloride against Mycoplasma hyopneumoniae infection. Ishag, Hassan Z. A.,Wu, Yu-zi,Liu, Mao-jun,Xiong, Qi-yan,Feng, Zhi-xin,Yang, Ruo-song,Shao, Guo-qing,Ishag, Hassan Z. A..

[14]The effects of Mycoplasma hyopneumoniae on porcine circovirus type 2 replication in vitro PK-15 cells. Wang, Haiyan,Feng, Zhixin,Wu, Yuzi,Wei, Yanna,Gan, Yuan,Hua, Lizhong,Li, Bin,Wang, Xiaomin,Liu, Maojun,Xiong, Qiyan,Shao, Guoqing,Wang, Haiyan,Feng, Zhixin,Shao, Guoqing.

[15]Use of serological and mucosal immune responses to Mycoplasma hyopneumoniae antigens P97R1, P46 and P36 in the diagnosis of infection. Feng, Zhi-Xin,Bai, Yun,Yao, Jing-Ting,Gan, Yuan,Wang, Hai-Yan,Wei, Yan-Na,Liu, Mao-Jun,Xiong, Qi-Yan,Bai, Fang-Fang,Li, Bin,Wu, Xu-Su,Shao, Guo-Qing,Feng, Zhi-Xin,Bai, Yun,Liu, Mao-Jun,Shao, Guo-Qing,Pharr, G. Todd,Wan, Xiu-Feng,Xiao, Shao-Bo,Chi, Ling-Zhi.

[16]Effects of Mycoplasma hyopneumoniae on porcine nasal cavity dendritic cells. Shen, Yumeng,Hu, Weiwei,Yang, Qian,Wei, Yanna,Feng, Zhixin.

[17]Transcription analysis on response of porcine alveolar macrophages to co-infection of the highly pathogenic porcine reproductive and respiratory syndrome virus and Mycoplasma hyopneumoniae. Li, Bin,Du, Luping,Xu, Xiangwei,Sun, Bing,Yu, Zhengyu,Feng, Zhixin,Liu, Maojun,Wei, Yanna,Wang, Haiyan,Shao, Guoqing,He, Kongwang,Li, Bin,Yu, Zhengyu,Feng, Zhixin,Liu, Maojun,Wei, Yanna,Wang, Haiyan,Shao, Guoqing,He, Kongwang.

作者其他论文 更多>>
置顶
购物车
反馈

© 京ICP备09089781号-29 主办单位：江苏省农业科学院

学术资源： 中国农业科学院 农业专业知识服务系统 农科联盟资源共建共享平台 中国农业科技文献与信息服务平台 中国农业期刊集成服务平台