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Molecular Cloning, Sequencing, and Expression of a l-Glutamine d-Fructose 6-Phosphate Amidotransferase Gene from Volvariella volvacea

文献类型： 外文期刊

作者： Luo, Chuping 1 ; Shao, Weilan 2 ; Li, Xun 2 ; Chen, Zhiyi 1 ; Liu, Yongfeng 1 ;

作者机构： 1.Jiangsu Acad Agr Sci, Inst Plant Protect, Nanjing 210014, Jiangsu, Peoples R China

2.Nanjing Normal Univ, Jiangsu Key Lab Biodivers & Biotechnol, Nanjing 210097, Jiangsu, Peoples R China

3.Nanjing Forestry Univ, Dept Bioengn, Nanjing 210017, Jiangsu, Peoples R China

关键词： L-Glutamine:D-fructose-6-phosphate amidotransferase;Volvariella volvacea;cDNA cloning;Heterologous expression

期刊名称：PROTEIN JOURNAL （ 影响因子：2.371； 五年影响因子：1.929 ）

ISSN： 1572-3887

年卷期： 2009 年 28 卷 1 期

页码：

收录情况： SCI

摘要： Using 3'-RACE and 5'-RACE, we have cloned and sequenced the genomic gene and complete cDNA encoding l-glutamine d-fructose 6-phosphate amidotransferase (GFAT) from the edible straw mushroom, Volvariella volvacea. Gfat contains five introns, and encodes a predicted protein of 697 amino acids that is homologous to other reported GFAT sequences. Southern hybridization indicated that a single gfat gene locus exists in the V. volvacea genome. Recombinant native V. volvacea GFAT enzyme, over-expressed using Escherichia coli and partially purified, had an estimated molecular mass of 306 kDa and consisted of four equal-sized subunits of 77 kD. Reciprocal plots revealed K (m) values of 0.55 and 0.75 mM for fructose 6-phosphate and l-glutamine, respectively. V. volvacea GFAT activity was inhibited by the end-product of the hexosamine pathway, UDP-GlcNAc, and by the glutamine analogues N (3)-(4-methoxyfumaroyl)-l-2,3-diaminopropanoic acid and 2-amino-2-deoxy-d-glucitol-6-phosphate.

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[1]Improved fruiting of the straw mushroom (Volvariella volvacea) on cotton waste supplemented with sodium acetate. Hou, Lijuan,Li, Yu,Chen, Mingjie,Li, Zhengpeng. 2017

[2]A Novel Soybean Intrinsic Protein Gene, GmTIP2;3, Involved in Responding to Osmotic Stress. Zhang, Dayong,He, Xiaolan,Xu, Zhaolong,Xu, Ling,Wei, Peipei,Huang, Yihong,Brestic, Marian,Ma, Hongxiang,Shao, Hongbo,Tong, Jinfeng,Brestic, Marian,Shao, Hongbo. 2016

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