文献类型： 外文期刊

作者： Chen, Shan ¹ ; Su, Yu-Wen ¹ ; Sun, Junjie ¹ ; Chen, Tingting ⁴ ; Zheng, Yuhao ¹ ; Sui, Lin-Jie ⁵ ; Yang, Shuangli ¹ ; Liu, Chenbin ⁶ ; Wang, Pengcheng ¹ ; Li, Tengfei ³ ; Chi, Qinghua ⁸ ; Sun, Hao ¹ ; Chen, Jinghu ¹ ; Xu, Bo-Qun ⁵ ; Huang, Zongxiong ¹⁰ ; Fang, Yimin ¹ ;

作者机构： 1.Nanjing Med Univ, Sch Pharm, Key Lab Cardiovasc & Cerebrovasc Med, Nanjing 211166, Peoples R China

2.Jiangsu Acad Agr Sci, Inst Agr Facil & Equipment, Key Lab Protected Agr Engn Middle & Lower Reaches, Minist Agr & Rural Affairs, Nanjing 210014, Peoples R China

3.Nanjing Med Univ, Sir Run Run Hosp, Dept Clin Pharmacol, Nanjing 211166, Peoples R China

4.Minjiang Teachers Coll, Sch Med Nursing, Outpatient Dept, Fuzhou 350108, Peoples R China

5.Nanjing Med Univ, Sir Run Run Hosp, Dept Gynecol & Obstet, Nanjing 211166, Peoples R China

6.Chinese Acad Med Sci & Peking Union Med Coll, Natl Canc Ctr, Dept Radiat Oncol, Natl Clin Res Ctr Canc,Canc Hosp, Shenzhen 518116, Peoples R China

7.Chinese Acad Med Sci & Peking Union Med Coll, Shenzhen Hosp, Shenzhen 518116, Peoples R China

8.Xiamen Univ, Sch Med, Affiliated Hosp 1, Nursing Teaching & Res Dept, Xiamen 361003, Peoples R China

9.Nanjing Med Univ, Dept Obstet & Gynecol, Affiliated Hosp 2, Nanjing 210011, Peoples R China

10.Fujian Fiber Inspect Ctr, Natl Textile & Garment Qual Supervis Testing Ctr, Fuzhou 350026, Peoples R China

关键词： single bacteria detection; label-free; morphological identification; convection; dark field scattering; microscopy

期刊名称：PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA （ 影响因子：11.1； 五年影响因子：12.0 ）

ISSN： 0027-8424

年卷期： 2022 年 119 卷 40 期

页码：

收录情况： SCI

摘要： Rapid detection of pathogenic bacteria within a few minutes is the key to control infec-tious disease. However, rapid detection of pathogenic bacteria in clinical samples is quite a challenging task due to the complex matrix, as well as the low abundance of bacteria in real samples. Herein, we employ a label-free single-particle imaging approach to address this challenge. By tracking the scattering intensity variation of single particles in free solu-tion, the morphological heterogeneity can be well identified with particle size smaller than the diffraction limit, facilitating the morphological identification of single bacteria from a complex matrix in a label-free manner. Furthermore, the manipulation of convec-tion in free solution enables the rapid screening of low-abundance bacteria in a small field of view, which significantly improves the sensitivity of single-particle detection. As a proof of concept demonstration, we are able to differentiate the group B streptococci (GBS)-positive samples within 10 min from vaginal swabs without using any biological reagents. This is the most rapid and low-cost method to the best of our knowledge. We believe that such a single-particle imaging approach will find wider applications in clinical diagnosis and disease control due to its high sensitivity, rapidity, simplicity, and low cost.