文献类型： 外文期刊

作者： Ling, Li-Li ¹ ; Lin, Hong-Hui ² ; Ji, Ben-Hua ² ; Jiao, De-Mao ² ;

作者机构： 1.Sichuan Univ, Coll Life Sci, Key Lab Bioresources & Ecoenvironm, Minist Educ, Chengdu 610064, Peoples R China

2.Sichuan Univ, Coll Life Sci, Key Lab Bioresources & Ecoenvironm, Minist Educ, Chengdu 610064, Peoples R China; Nantong Univ, Inst Life Sci, Nantong 510061, Peoples R China; Jiangsu Acad Agr Sci, Nanjing 210014, Peoples R China

关键词： anther culture;phosphoenolpyruvate carboxylase (PEPC);physiological inherited trait;rice hybrid;transgenic rice

期刊名称：JOURNAL OF INTEGRATIVE PLANT BIOLOGY （ 影响因子：7.061； 五年影响因子：6.002 ）

ISSN： 1672-9072

年卷期： 2006 年 48 卷 12 期

页码：

收录情况： SCI

摘要： To elucidate the photosynthetic physiological characteristics and the physiological inherited traits of rice (Oryza sativa L.) hybrids and their parents, physiological indices of photosynthetic CO2 exchange and chlorophyll fluorescence parameters were measured in leaves of the maize phosphoenolpyruvate carboxylase (PEPC) transgenic rice as the male parent, sp. japonica rice cv. 9516 as the female parent, and the stable JAAS45 pollen line. The results revealed that the PEPC gene could be stably inherited and transferred from the male parent to the JAAS45 pollen line. Moreover, the JAAS45 pollen line exhibited high levels of PEPC activity, manifesting higher saturated photosynthetic rates, photosynthetic apparent quantum yield (AQY), photochemical efficiency of photosystem II and photochemical and non-photochemical quenching, which indicated that the JAAS45 pollen line has a high tolerance to photo-inhibition/photo-oxidation under strong light and high temperature. Furthermore, JAAS45 was confirmed to still be a C-3 plant by delta C-13 carbon isotope determination and was demonstrated to have a limited photosynthetic C-4 microcycle by feeding with exogenous C-4 primary products, such as oxaloacetate or malate, or phosphoenolpyruvate. The present study explains the physiological inherited properties of PEPC transgenic rice and provides an expectation for the integration of traditional breeding and biological technology.