RAPD profiling in detecting genetic variation in endemic Coelonema (Brassicaceae) of Qinghai-Tibet Plateau of China
文献类型： 外文期刊
作者： Chen, SL 1 ; Xia, T 2 ; Chen, SY 2 ; Zhou, YJ 2 ;
作者机构： 1.Chinese Acad Sci, NW Plateau Inst Biol, Lab Qinghai Tibet Biol evolut & Adaptat, Xining Qinghai 810001, Peoples R China
2.Chinese Acad Sci, NW Plateau Inst Biol, Lab Qinghai Tibet Biol evolut & Adaptat, Xining Qinghai 810001, Peoples R China; Jiangsu Acad Agr Sci, Nanjing 210014, Peoples R China
关键词： Coelonema;genetic variation;RAPD;Qinghai-Tibet Plateau
期刊名称：BIOCHEMICAL GENETICS （ 影响因子：1.89； 五年影响因子：1.959 ）
ISSN： 0006-2928
年卷期： 2005 年 43 卷 3-4 期
页码：
收录情况： SCI
摘要： Random amplified polymorphic DNA ( RAPD) markers were used to measure genetic diversity of Coelonema draboides ( Brassicaceae), a genus endemic to the Qilian Mountains of the Qinghai-Tibet Plateau. We sampled 90 individuals in 30 populations of Coelonema draboides from Datong and Huzhu counties of Qinghai Province in P. R. China. A total of 186 amplified bands were scored from the 14 RAPD primers, with a mean of 13.3 amplified bands per primer, and 87% ( 161 bands) polymorphic bands (PPB) was found. Analysis of molecular variance (AMOVA) shows that a large proportion of genetic variation (84.2%) resides among individuals within populations, while only 15.8% resides among populations. The species shows higher genetic diversity between individuals than other endemic and endangered plants. The RAPDs provide a useful tool for assessing genetic diversity of rare, endemic species and for resolving relationships among populations. The results show that the genetic diversity of this species is high, possibly allowing it to adapt more easily to environmental variations. The main factor responsible for the high level of differentiation within populations and the low level of diversity among populations is probably the outcrossing and long-lived nature of this species. Some long-distance dispersal, even among far separated populations, is also a crucial determinant for the pattern of genetic variation in the species. This distributive pattern of genetic variation of C. draboides populations provides important baseline data for conservation and collection strategies for the species. It is suggested that only populations in different habitats should be studied and protected, not all populations, so as to retain as much genetic diversity as possible.
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