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A limited photosynthetic C-4-microcycle and its physiological function in transgenic rice plant expressing the maize PEPC gene

文献类型： 外文期刊

作者： Ji, BH 1 ; Zhu, SQ 2 ; Jiao, DM 2 ;

作者机构： 1.Jiangsu Acad Agr Sci, Inst Agrobiol Genet & Physiol, Nanjing 210014, Peoples R China

2.Jiangsu Acad Agr Sci, Inst Agrobiol Genet & Physiol, Nanjing 210014, Peoples R China; Nantong Normal Coll, Dept Life Sci & Technol, Nantong 226007, Peoples R China

关键词： transgenic rice;photosynthetic C4-microcycle;chlorophyll a fluorescence;phosphoenolpyruvate carboxylase (PEPC);C-4-bicarboxylate

期刊名称：ACTA BOTANICA SINICA （ 影响因子：0.599； ）

ISSN： 0577-7496

年卷期： 2004 年 46 卷 5 期

页码：

收录情况： SCI

摘要： Photosynthetic C-4-microcycle and its function in different genotype rices were explored comparatively using PEPC transgenic rice and homozygous wild genotype (WT) rice (Oryza sativa L. subsp.japonica Kitaake) as experimental material. In untransformed WT, there existed an intact C-4 photosynthetic enzyme system detected by the activities of enzymes of photosynthetic C4 pathway, i.e. phosphoenolpyruvate carboxylase (PEPC), NADP(+)-malic enzyme (NADP(+)-ME), NADP(+)-malate dehydrogenase (NADP(+)-MDH), pyruvate orthophosphate dikinase (PPDK), and indicating that there is a primitive photosynthetic C-4-pathway with increased photosynthetic rate in leaf discs or chloroplasts fed with exogenous oxaloacetate (OAA) or malate (MA). Furthermore, photosynthetic C-4 microcycle was promoted in a great range in transgenic rice introduced a maize specific PEPC gene. Enhancement of photosynthetic C-4-microcycle further played some role in raising the net photosynthetic rates (Pn) and debasing the ratio of Pr/Pn by comparing the CO2 gas exchange rates in different genotype rices, WT rice and PEPC transgenic rice. Analyzing the chlorophyll a fluorescence characteristics showed that increase of photosynthetic C-4-microcycle companied with the raising PS II maximum photochemical efficiency (Fv/Fm) and photochemical quenching (qP), and the lowering of non-photochemical quenching (qN). These results will provide scientific evidence for genetic breeding to improve photosynthetic efficiency in crops by gene engineering.

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