文献类型： 外文期刊
作者： Lu, Z 1 ; Huang, M 2 ; Ge, DP 2 ; Yang, YH 2 ; Cao, XN 2 ; Qin, P 2 ; She, JM 2 ;
作者机构： 1.Nanjing Univ, State Key Lab Pharmaceut Biotechnol, Plant Cell Physiol & Mol Biol Lab, Dept Biol Sci & Technol,Coll Life Sci, Nanjing 210093, Peoples R China
2.Nanjing Univ, State Key Lab Pharmaceut Biotechnol, Plant Cell Physiol & Mol Biol Lab, Dept Biol Sci & Technol,Coll Life Sci, Nanjing 210093, Peoples R China; Jiangsu Acad Agr Sci, Inst Agrobiol Genet & Physiol, Nanjing 210014, Peoples R China
关键词： 6-benzylaminopurine;brassinolide;callus;indole-3-acetic acid;regeneration;Spartina patens
期刊名称：PLANT CELL TISSUE AND ORGAN CULTURE （ 影响因子：2.711； 五年影响因子：2.73 ）
ISSN： 0167-6857
年卷期： 2003 年 73 卷 1 期
页码：
收录情况： SCI
摘要： The effect of brassinolide (BL) on cultured calluses of Spartina patens (Ait.) Muhl. (Poaceae), a halophyte monocot was studied. BL at 0.03-0.04 mg l(-1) at fixed concentrations of IAA (0.2 mg l(-1)) and BA (3.0 mg l(-1)) added in MS medium increased the ratio for fresh weight (CIRFW) to dry weight (CIRDW) by 96-111% and 235-326%. Similarly, in callus regeneration capacity, BL at 0.03 mg l(-1) was most effective, increasing the shoot regeneration ratio (SRR) by 425%. BL at 0.04 mg l(-1) had not such an increasing effect as BL at 0.03 mg l(-1), which increased SRR by 79%. However, BL at 0.005 mg l(-1) promoted regenerated shoot growth most significantly, increasing the shoot height increasing ratio (SHIR) by 395% after a 40-day culture. BL at 0.05 mg l(-1) was least effective in the callus regeneration and regenerated shoot growth, decreasing SRR by 27% and SHIR by 52%. Present results suggest that BL at 0.03 mg l(-1) is suitable for the callus growth and shoot regeneration, while BL at 0.005 mg l(-1) effectively enhanced the regenerated shoot growth.
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