Correlation between appearance of embryogenic cells and the IAA levels in rice somatic cell culture
文献类型： 外文期刊
作者： Chen, YF 1 ; Zhou, X 2 ; Tang, RS 2 ; Zhang, JY 2 ; Mei, CS 2 ;
作者机构： 1.Nanjing Agr Univ, Dept Agron, Nanjing 210095, Peoples R China
2.Nanjing Agr Univ, Dept Agron, Nanjing 210095, Peoples R China; Jiangsu Acad Agr Sci, Inst Agrobiol Genet & Physiol, Nanjing 210014, Peoples R China
关键词： rice;embryogenic cell;IAA;2,4-D;2,3,5-triiodobenzoic acid
期刊名称：ACTA BOTANICA SINICA （ 影响因子：0.599； ）
ISSN： 0577-7496
年卷期： 1998 年 40 卷 5 期
页码：
收录情况： SCI
摘要： Changes of endogenous IAA level and IAA action in cultured rice (Oryza saliva L.) somatic cells during the period from 7th to 15th day which was the transition from somatic to embryogenic cells were observed, The study was carried out in three experimental systems viz. mature caryopsis and young panicles (2 similar to 5 mm long) of rice cv. "Guangluai 4" under normal osmosis (3% sucrose), mature caryopses from rice cv. "Yanjing 2" or "Guangluai 4" under normal and higher osmosis (5% sucrose or 2.5% sorbitol). During this period, endogenous IAA contents were greatly increased in young-panicle calli under normal osmosis and mature-caryoptic calli under higher osmosis but decreased in mature-caryoptic calli under normal osmosis, Exogenous IAA could induce the appearance of embryogenic cell from nonembryogenic callus at a lower frequency. And 2,3,5-tri-iodobenzoic acid could increase the frequency of embryogenic cell induction. From these results it could be concluded that accumulation of higher IAA level in the cultured rice cells was essential for induction of embryogenic cell appearance, Since 2,4-D was involved in all induction medium with the same concentration but exerted different effects on embryogenic cell induction, it was suggested that it might act through mediating the endogenous IAA metabolism.
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