CRISPR/Cas9-mediated methoprene-tolerant 1 knockout results in precocious metamorphosis of beet armyworm (Spodoptera exigua) only at the late larval stage
文献类型： 外文期刊
作者： Zhao, Jing 1 ; Tan, Yongan 1 ; Jiang, Yiping 1 ; Zhu-Salzman, Keyan 3 ; Xiao, Liubin 1 ;
作者机构： 1.Jiangsu Acad Agr Sci, Inst Plant Protect, Nanjing, Peoples R China
2.Jiangsu Acad Agr Sci, Key Lab Food Qual & Safety Jiangsu Prov, State Key Lab Breeding Base, Nanjing, Peoples R China
3.Texas A&M Univ, Coll Agr & Life Sci, Dept Entomol, College Stn, TX USA
关键词： CRISPR; Cas9; metamorphosis; S; exigua; SeMet
期刊名称：INSECT MOLECULAR BIOLOGY （ 影响因子：3.424； 五年影响因子：3.268 ）
ISSN： 0962-1075
年卷期：
页码：
收录情况： SCI
摘要： Juvenile hormone (JH) controls almost every aspect of an insect, especially metamorphosis. Since RNA interference works on transcripts and is often insufficient in Lepidoptera, how JH affects larval development in these insects is not well studied. Using the CRISPR/Cas9 technique, we knocked out Spodoptera exigua methoprene-tolerant 1 (SeMet1) gene of beet armyworm by modifying two sites in the coding region. However, SeMet1 knockout did not affect egg hatch rate or larval development at L1-L3 stages. In contrast to the consistent five larval instars of the control group, L4 SeMet1 mutants began to show signs of precocious metamorphosis, that is, small patches of pupal cuticle. Most L4 and all L5 SeMet1 mutants died for failing to shed their mosaic cuticles. RNA-seq indicated that most genes encoding pupal cuticle proteins and chitinase genes were altered in SeMet1 mutant L4 larvae. SeKr-h1, a key transcription factor in JH action was significantly down-regulated in L3-L5 larvae, while SeBR-C, a pupal indicator was only upregulated in L4-L5 larvae. These results suggested that S. exigua larvae may initially develop independently of JH, and involve SeMet1 in transducing JH signalling, leading to controlled larval metamorphosis at the late larval stage. We believe our findings will enhance better understanding of JH regulation of larval development.
- 相关文献
作者其他论文 更多>>
-
Biosynthesis of sakuranetin regulated by OsMPK6-OsWRKY67-OsNOMT cascade enhances resistance to false smut disease
作者：Ma, Jinbiao;Wei, Lirong;Huang, Keyi;Wang, Dacheng;Gao, Jiameng;Chen, Xi;Guo, Huimin;Gao, Shangyu;Zhang, Min;Li, Shujing;Yu, Chenjie;Zhao, Jing;Wu, Jingni;Gu, Qin;Wang, Yiming;Kim, Sun Tae;Gupta, Ravi;Xiong, Guosheng;Lo, Clive;Liu, Yongfeng
关键词：immune signaling; rice; rice false smut disease; sakuranetin; WRKY transcription factor
-
Comparative toxicity mechanisms of sulfoxaflor and lambda-cyhalothrin against Apolygus lucorum from enzymatic and transcriptomic perspectives: Efficient application of insecticides
作者：Xu, Lu;Liu, Baosheng;Xiao, Liubin;Zhang, Zhichun;Niu, Hongtao;Zhao, Dongxiao;Sun, Shuai;Guo, Huifang;Xu, Lu
关键词：Apolygus lucorum; Sulfoxaflor; Lambda-cyhalothrin; Transcriptome analysis; Toxicity difference; Enzyme activity
-
pH-responsive nanoparticles for oral delivery of RNAi for sustained protection against Spodoptera exigua
作者：Jiang, Yiping;Zong, Suman;Zhao, Jing;Xiao, Liubin;Xu, Dejin;Xu, Guangchun;Tan, Yongan;Wang, Xiaofeng;Zhu-Salzman, Keyan
关键词：Spodoptera exigua; RNAi; Pest management; Nanocarrier
-
Nano-delivery platform with strong protection and efficient delivery: preparation of self-assembled RNA pesticide with dual RNAi targets against Apolygus lucorum
作者：Qiao, Heng;Zhao, Jing;Xiao, Liubin;Xu, Dejin;Xu, Guangchun;Tan, Yongan;Jiang, Qinhong;Shen, Jie;Yan, Shuo;Jiang, Qinhong;Shen, Jie;Yan, Shuo;Hao, Dejun;Zhu-Salzman, Keyan;Gu, Aiguo
关键词：
Apolygus lucorum ; Delivery system; Nanocarrier; RNA interference; RNA pesticide -
Methuselah-like 2 mediated 20-hydroxyecdysone (20E) signaling regulates molting and fecundity in Nilaparvata lugens (Stål) (Hemiptera: Delphacidae)
作者：Zhao, Xudong;Dong, Qiaoqiao;Zhu, Haowen;Ding, Yuting;Deng, Di;Ge, Linquan;Miao, Hong;Tan, Yongan
关键词：
Nilaparvata lugens St & aring;l; Methuselah-like 2; 20E signaling; metamorphosis; molting; fecundity -
A Wolbachia strain in the flour moth, Ephestia kuehniella, causes moderate cytoplasmic incompatibility but has little impact on host fitness
作者：Zhao, Jing;Thia, Joshua A.;Yang, Qiong;Kaur, Jasmeen;Qazi, Hareem;Ross, Perran A.;Hoffmann, Ary A.;Zhao, Jing
关键词：Wolbachia; Phylogeny; Flour moth; Cytoplasmic incompatibility; Fitness costs; Inbreeding
-
Improving the Surface Color and Delaying Softening of Peach by Minimizing the Harmful Effects of Ethylene in the Package
作者：Zhou, Hongsheng;Zhang, Yingtong;Ling, Jun;Li, Pengxia;Zhou, Hongsheng;Zhao, Jing;Gao, Ying;Zhou, Qian;Zhou, Hongsheng;Ma, Siyu;Huang, Wen
关键词：peach; modified atmosphere packaging; ethylene absorbent; firmness; anthocyanin
