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Proteomic analysis of elite soybean Jidou17 and its parents using iTRAQ-based quantitative approaches

文献类型: 外文期刊

作者: Qin, Jun 1 ; Gu, Feng 1 ; Liu, Duan; Yin, Changcheng; Zhao, Shuangjin 1 ; Chen, Hao; Zhang, Jianan; Yang, Chu 1 ;

作者机构: 1.Hebei Acad Agr & Forestry Sci, North China Key Lab Soybean Biol & Genet Improvem, Natl Soybean Improvement Ctr Shijiazhuang Subctr, Minist Agr,Cereal & Oil Crop Inst, Shijiazhuang 050031, Peoples R China

2.Hebei Acad Agr & Forestry Sci, North China Key Lab Soybean Biol & Genet Improvem, Natl Soybean Improvement Ctr Shijiazhuang Subctr, Minist Agr,Cereal & Oil Crop Inst, Shijiazhuang 050031, Peoples R China;

关键词: Soybean;iTRAQ;Proteomic

期刊名称:PROTEOME SCIENCE ( 影响因子:2.48; 五年影响因子:2.619 )

ISSN: 1477-5956

年卷期: 2013 年 11 卷

页码:

收录情况: SCI

摘要: Background: Derived from Hobbit as the female parent and Zao5241 as the male parent, the elite soybean cultivar Jidou17 is significantly higher yielding and shows enhanced qualities and stronger resistance to non-biological stress than its parents. The purpose of this study is to understand the difference in protein expression patterns between Jidou17 and its parental strains and to evaluate the parental contributions to its elite traits. Results: Leaves (14 days old) from Jidou17 and its parental cultivars were analysed for differential expressed proteins using an iTRAQ-based (isobaric tags for relative and absolute quantitation) method. A total of 1269 proteins was detected, with 141 and 181 proteins in Jidou17 differing from its female and male parent, respectively. Functional classification and an enrichment analysis based on biological functions, biological processes, and cellular components revealed that all the differential proteins fell into many functional categories but that the number of proteins varied greatly for the different categories, with enrichment in specific categories. A pathway analysis indicated that the differentiated proteins were mainly classified into the ribosome assembly pathway. Protein expression clustering results showed that the expression profiles between Jidou17 and its female parent Hobbit were more similar than those between Jidou17 and its male parent Zao5241 and between the two parental strains. Therefore, the female parent Hobbit contributed more to the Jidou17 genotype. Conclusions: This study applied a proven technique to study proteomics in 14-day-old soybean leaves and explored the depth and breadth of soybean protein research. The results provide new data for further understanding the mechanisms of elite cultivar development.

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