Proteomic analysis of elite soybean Jidou17 and its parents using iTRAQ-based quantitative approaches
文献类型: 外文期刊
作者: Qin, Jun 1 ; Gu, Feng 1 ; Liu, Duan; Yin, Changcheng; Zhao, Shuangjin 1 ; Chen, Hao; Zhang, Jianan; Yang, Chu 1 ;
作者机构: 1.Hebei Acad Agr & Forestry Sci, North China Key Lab Soybean Biol & Genet Improvem, Natl Soybean Improvement Ctr Shijiazhuang Subctr, Minist Agr,Cereal & Oil Crop Inst, Shijiazhuang 050031, Peoples R China
2.Hebei Acad Agr & Forestry Sci, North China Key Lab Soybean Biol & Genet Improvem, Natl Soybean Improvement Ctr Shijiazhuang Subctr, Minist Agr,Cereal & Oil Crop Inst, Shijiazhuang 050031, Peoples R China;
关键词: Soybean;iTRAQ;Proteomic
期刊名称:PROTEOME SCIENCE ( 影响因子:2.48; 五年影响因子:2.619 )
ISSN: 1477-5956
年卷期: 2013 年 11 卷
页码:
收录情况: SCI
摘要: Background: Derived from Hobbit as the female parent and Zao5241 as the male parent, the elite soybean cultivar Jidou17 is significantly higher yielding and shows enhanced qualities and stronger resistance to non-biological stress than its parents. The purpose of this study is to understand the difference in protein expression patterns between Jidou17 and its parental strains and to evaluate the parental contributions to its elite traits. Results: Leaves (14 days old) from Jidou17 and its parental cultivars were analysed for differential expressed proteins using an iTRAQ-based (isobaric tags for relative and absolute quantitation) method. A total of 1269 proteins was detected, with 141 and 181 proteins in Jidou17 differing from its female and male parent, respectively. Functional classification and an enrichment analysis based on biological functions, biological processes, and cellular components revealed that all the differential proteins fell into many functional categories but that the number of proteins varied greatly for the different categories, with enrichment in specific categories. A pathway analysis indicated that the differentiated proteins were mainly classified into the ribosome assembly pathway. Protein expression clustering results showed that the expression profiles between Jidou17 and its female parent Hobbit were more similar than those between Jidou17 and its male parent Zao5241 and between the two parental strains. Therefore, the female parent Hobbit contributed more to the Jidou17 genotype. Conclusions: This study applied a proven technique to study proteomics in 14-day-old soybean leaves and explored the depth and breadth of soybean protein research. The results provide new data for further understanding the mechanisms of elite cultivar development.
- 相关文献
作者其他论文 更多>>
-
Identification of QTLs and candidate genes for water-soluble protein content in soybean seeds
作者:Zhang, Xujuan;Wang, Fengmin;Chen, Qiang;Zhao, Qingsong;Zhao, Tiantian;Hu, Xuejie;Liu, Luping;Qi, Jin;Zhang, Mengchen;Yang, Chunyan;Qin, Jun;Zhang, Xujuan;Hu, Xuejie;Qiao, Yake;Zhao, Tiantian;Qi, Jin
关键词:Soybean; WSP; QTL; Candidate gene mining
-
Dissecting the role of soybean rhizosphere-enriched bacterial taxa in modulating nitrogen-cycling functions
作者:Wang, Tianshu;Gao, Miao;Shao, Weiwei;Wang, Li;Yao, Shuihong;Yang, Chunyan;Wang, Xing;Zhang, Bin
关键词:Soybean rhizosphere effect; N cycle; Rhizosphere-enriched taxa; Shared taxa; Site- and variety-specific taxa
-
A comprehensive analysis of the WRKY family in soybean and functional analysis of GmWRKY164-GmGSL7c in resistance to soybean mosaic virus
作者:Zhao, Zhihua;Wang, Rongna;Su, Weihua;Sun, Tianjie;Qi, Mengnan;Zhang, Xueyan;Wei, Fengju;Zhang, Jie;Wang, Dongmei;Yu, Zhouliang;Xiao, Fuming;Yan, Long;Yang, Chunyan
关键词:Soybean; SMV; WRKY gene family; Callose deposition
-
Cold tolerance SNPs and candidate gene mining in the soybean germination stage based on genome-wide association analysis
作者:Chen, Yuehan;Ma, Qian;Chen, Yuehan;Liu, Zhi;Yang, Qing;Li, Chenhui;Shi, Xiaolei;Zhang, Mengchen;Yang, Chunyan;Yan, Long;Han, Dezhi;Jia, Hongchang;Wang, Shu;Lu, Wencheng;Qiu, Lijuan
关键词:
-
De novo genome assembly of a high-protein soybean variety HJ117
作者:Liu, Zhi;Yang, Qing;Liu, Bingqiang;Li, Chenhui;Shi, Xiaolei;Wei, Yu;Yang, Chunyan;Zhang, Mengchen;Yan, Long;Li, Chenhui;Guan, Yuefeng
关键词:Soybean; De novo assembly; Genome feature; High protein content
-
Ability of Genomic Prediction to Bi-Parent-Derived Breeding Population Using Public Data for Soybean Oil and Protein Content
作者:Li, Chenhui;Li, Chenhui;Yang, Qing;Liu, Bingqiang;Shi, Xiaolei;Liu, Zhi;Yang, Chunyan;Zhang, Mengchen;Yan, Long;Wang, Tao;Xiao, Fuming;Shi, Ainong
关键词:soybean; protein content; oil content; GP; prediction ability; G-BLUP
-
Identification of Putative Quantitative Trait Loci for Improved Seed Oil Quality in Peanuts
作者:Hu, Pengju;Zhang, Jianan;Song, Yahui;Zhao, Xing;Jin, Xinxin;Su, Qiao;Yang, Yongqing;Wang, Jin
关键词:peanut; oil quality; fatty acids; QTL; epistatic effects