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Supercritical fluid extraction of paeonol from Cynanchum paniculatum (Bge.) Kitag. and subsequent isolation by high-speed counter-current chromatography coupled with high-performance liquid chromatography-photodiode array detector

文献类型： 外文期刊

作者： Sun, Yinshi 1 ; Liu, Zhengbo 1 ; Wang, Jianhua 1 ; Tian, Wei 3 ; Zhou, Hongying 1 ; Zhu, Lixiang 1 ; Zhang, Chunling 1 ;

作者机构： 1.Shandong Agr Univ, Coll Agron, Tai An 271018, Shandong, Peoples R China

2.Shandong Agr Univ, State Key Lab Crop Biol, Tai An 271018, Shandong, Peoples R China

3.Hebei Acad Agr & Forestry Sci, Herbal Med Res Ctr, Shijiazhuang 050051, Peoples R China

关键词： Paeonol;Supercritical fluid extraction (SFE);High-speed counter-current chromatography (HSCCC);Cynanchum paniculatum (Bge.) Kitag

期刊名称：SEPARATION AND PURIFICATION TECHNOLOGY （ 影响因子：7.312； 五年影响因子：6.656 ）

ISSN： 1383-5866

年卷期： 2008 年 64 卷 2 期

页码：

收录情况： SCI

摘要： Supercritical fluid extraction (SFE) was used to extract paeonol from Cynanchum paniculatum (Bge.) Kitag. The extraction conditions including pressure, temperature, time and sample particle size were optimized with an orthogonal test. Under the optimized conditions of 15 MPa, 55 degrees C, 1.5 h and a sample particle size of 40-60 mesh, the process was repeated 10 times, and the extract was then collected. The yield of the crude extract from preparative SFE was 6.02%, which contained paeonol 72.02%. Paeonol of crude extract obtained by SFE, Ultrasonic Extraction (UE), Microwave-Assisted :MA), Steam Distillation (SD) and Soxhlet Extraction (SE) were further analyzed by HPLC-PDA to compare the extraction methods, and SFE is considered as the optimum one among the five processes. The SFE extract obtained was then purified successfully by high-speed counter-current chromatography (HSCCC) with a two-phase solvent system composed of petroleum ether-ethyl acetate-methanol-water (2:6:3:4,v/v) in one-step separation. From 2.42 g of the crude extract, 1.07 g of paeonol was obtained at 99.4% purity as determined by HPLC-PDA. These results indicate that SFE and HSCCC are very powerful techniques for the extraction and purification of paeonol from C. paniculatum. (C) 2008 Elsevier B.V. All rights reserved.

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