An intronless sucrose:fructan-6-fructosyltransferase (6-SFT) gene from Dasypyrum villosum enhances abiotic tolerance in tobacco
文献类型： 外文期刊
作者： He, X. L. 1 ; Wang, J. W. 3 ; Li, W. X. 4 ; Chen, Z. Z. 1 ; Wu, J. 1 ; Zhao, J. X. 1 ; Su, J. N. 1 ; Wang, Z. H. 1 ; Chen, X 1 ;
作者机构： 1.Northwest A&F Univ, Coll Agron, Yangling 712100, Shaanxi, Peoples R China
2.Shaanxi Key Lab Genet Engn Plant Breeding, Yangling 712100, Shaanxi, Peoples R China
3.Kaili Univ, Coll Environm & Life Sci, Kaili 556011, Guizhou, Peoples R China
4.Henan Acad Agr Sci, Inst Wheat Res, Zhengzhou 450002, Henan, Peoples R China
关键词： Agrobacterium tumefaciens;drought;freezing;Nicotiana tabacum;salinity;transgenic plants
期刊名称：BIOLOGIA PLANTARUM （ 影响因子：1.747； 五年影响因子：2.146 ）
ISSN： 0006-3134
年卷期： 2017 年 61 卷 2 期
页码：
收录情况： SCI
摘要： Fructans play vital roles in enhancing plant abiotic stress tolerance by reducing oxidative damage, stabilizing cell membranes, improving the osmotic adjustment capacity, and lowering the freezing point. In this study, a sucrose: fructan-6-fructosyltransferase (6-SFT) gene involved in the synthesis of fructans was isolated from Dasypyrum villosum, Dv-6-SFT, using genomic walking and reverse transcription (RT)-PCR. Alignment of the cDNA sequence with its genomic counterpart showed that no introns were present in the Dv-6-SFT gene, and thus it differs from all other plant 6-SFTs that have been cloned previously. Sequence analysis showed that the cDNA of the Dv-6-SFT sequence comprised 2 175 bp with a 1 863 bp open reading frame, and its deduced protein comprised 620 amino acids with a predicted molecular mass of 68.47 kDa. The Dv-6-SFT gene was transferred into tobacco (Nicotiana tabacum L.) cv. W38 via Agrobacterium-mediated transformation. The screened plants were tested by PCR and semi-quantitative RT-PCR, and the transgenic plants were evaluated under drought, cold, and salt stresses. The Dv-6-SFT transgenic tobacco plants had higher resistance to drought, cold, and salt stress than the non-transgenic plants. Further analysis showed that the transgenic plant expressing Dv-6-SFT had increased content of saccharides and proline, but reduced content of malondialdehyde in leaves. The results of this study demonstrate that the Dv-6-SFT gene is a potential candidate for conferring abiotic stress tolerance in plants and it could be used in crop improvement breeding programs.
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