文献类型： 外文期刊
作者： Hu, Jinlu 1 ; Li, Tianpei 2 ; Xu, Wen 4 ; Zhan, Jiao 2 ; Chen, Hui 2 ; He, Chenliu 2 ; Wang, Qiang 5 ;
作者机构： 1.Northwestern Polytech Univ, Sch Life Sci, Xian, Peoples R China
2.Chinese Acad Sci, Inst Hydrobiol, Key Lab Algal Biol, Wuhan, Peoples R China
3.Univ Chinese Acad Sci, Beijing, Peoples R China
4.Henan Acad Agr Sci, Crop Designing Ctr, Zhengzhou, Peoples R China
5.Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan, Peoples R China
关键词： sRNA;RbIR;RuBisCo;rbcL;Synechocystis sp PCC 6803
期刊名称：FRONTIERS IN MICROBIOLOGY （ 影响因子：5.64； 五年影响因子：6.32 ）
ISSN： 1664-302X
年卷期： 2017 年 8 卷
页码：
收录情况： SCI
摘要： Small regulatory RNAs (sRNAs) function as transcriptional and post-transcriptional regulators of gene expression in organisms from all domains of life. Cyanobacteria are thought to have developed a complex RNA-based regulatory mechanism. In the current study, by genome-wide analysis of differentially expressed small RNAs in Synechocystis sp. PCC 6803 under high light conditions, we discovered an asRNA (RblR) that is 113nt in length and completely complementary to its target gene rbcL, which encodes the large chain of RuBisCO, the enzyme that catalyzes carbon fixation. Further analysis of the RbIR(+)/(-) mutants revealed that RblR acts as a positive regulator of rbcL under various stress conditions; Suppressing RbIR adversely affects carbon assimilation and thus the yield, and those phenotypes of both the wild type and the overexpressor could be downgraded to the suppressor level by carbonate depletion, indicated a regulatory role of RblR in CO2 assimilation. In addition, a real-time expression platformin Escherichia coli was setup and which confirmed that RbIR promoted the translation of the rbcL mRNA into the RbcL protein. The present study is the first report of a regulatory RNA that targets RbcL in Synechocystis sp. PCC 6803, and provides strong evidence that RbIR regulates photosynthesis by positively modulating rbcL expression in Synechocystis.
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