Functional Characterization of 9-/13-LOXs in Rice and Silencing Their Expressions to Improve Grain Qualities
文献类型： 外文期刊
作者： RoyChowdhury, Moytri 1 ; Li, Xiaobai 2 ; Qi, Hangying 4 ; Li, Wenxu 5 ; Sun, Jian 2 ; Huang, Cheng 4 ; Wu, Dianxing 2 ;
作者机构： 1.Washington State Univ, Dept Hort, Pullman, WA 99164 USA
2.Zhejiang Univ, Int Atom Energy Agcy Collaborating Ctr, State Key Lab Rice Biol, Hangzhou 310029, Zhejiang, Peoples R China
3.Zhejiang Acad Agr Sci, Hangzhou 310021, Zhejiang, Peoples R China
4.Shaoxing & Zhuji Agr Bur, Agr Extens Extending Stn, Shaoxing 312000, Zhejiang, Peoples R China
5.Henan Acad Agr Sci, Inst Wheat Res, Zhengzhou 450002, Henan, Peoples R China
期刊名称：BIOMED RESEARCH INTERNATIONAL （ 影响因子：3.411； 五年影响因子：3.62 ）
ISSN： 2314-6133
年卷期： 2016 年
页码：
收录情况： SCI
摘要： Lipoxygenases (LOXs) are involved in oxidative rancidity and render rice unsuitable for human consumption. Here, RNA interference- (RNAi-) induced gene expression inhibition was used to analyze the functions of the bran/seed-specific LOXs in rice. r9-LOX1 and L-2 (9-LOX category) were the candidate genes expressing a bran/seed-specific LOX, while RCI-1 was (13-LOX category) a plastid-specific LOX. Real-time PCR showed that three LOXs were cultivar/tissue specific expression on a certain level. r9-LOX1 and L-2 were generally much higher in active bran/seed than in stabilized bran, mature seed, and regenerated plant. RCI-1 was barely expressed in seed. In transgenic lines, r9-LOX1, as well as L-2, expression was dramatically downregulated, compared to the nontransgenic controls. SPME/GC-MS analysis of r9-LOX1 RNAi transgenic lines showed 74.33% decrease in nonanal content (formed during oxidation of linoleic acid by lipoxygenase), but 388.24% increase in acetic acid and 184.84% hexanal (direct products of 13-LOX). These results indicate that r9-LOX1 positively regulates the amount of nonanal but negatively regulates acetic acid and hexanal. The negative regulation may be due to a mechanism of negative feedback between LOX family members. The information will help comprehensively understand the function of the bran/seed-specific LOXs, r9-LOX1, and improve the storage quality in the future.
- 相关文献
作者其他论文 更多>>
-
Genetic dissection of grain iron concentration in hexaploid wheat (Triticum aestivum L.) using a genome-wide association analysis method
作者：Wang, Jiansheng;Lei, Zhensheng;Wang, Jiansheng;Shi, Xia;Zhou, Zhengfu;Qin, Maomao;Wang, Yahuan;Li, Wenxu;Yang, Pan;Wu, Zhengqing;Lei, Zhensheng;Wang, Jiansheng
关键词：Wheat; Grain iron; QTL; GWAS; SNP
-
Promoter DNA hypermethylation of TaGli-gamma-2.1 positively regulates gluten strength in bread wheat
作者：Zhou, Zhengfu;Liu, Congcong;Qin, Maomao;Li, Wenxu;Hou, Jinna;Shi, Xia;Dai, Ziju;Lei, Zhensheng;Wu, Zhengqing;Liu, Congcong;Yao, Wen;Lei, Zhensheng;Li, Yang;Zhou, Zhengfu;Tian, Baoming;Lei, Zhensheng;Wu, Zhengqing
关键词：Wheat; Gluten strength; Gliadin; TaGli-gamma-2.1; DNA methylation
-
Dissection of the Genetic Architecture for Quantities of Gliadins Fractions in Wheat (Triticum aestivum L.)
作者：Zhou, Zhengfu;Geng, Shenghui;Guan, Huiyue;Liu, Congcong;Qin, Maomao;Li, Wenxu;Shi, Xia;Dai, Ziju;Lei, Zhensheng;Wu, Zhengqing;Hou, Jinna;Zhou, Zhengfu;Geng, Shenghui;Guan, Huiyue;Lei, Zhensheng;Wu, Zhengqing;Yao, Wen;Lei, Zhensheng
关键词：QTL mapping; gliadin content; KASP marker; wheat; end-use quality
-
Genome-wide association study reveals the genetic architecture for calcium accumulation in grains of hexaploid wheat (Triticum aestivum L.)
作者：Shi, Xia;Zhou, Zhengfu;Li, Wenxu;Qin, Maomao;Yang, Pan;Hou, Jinna;Lei, Zhensheng;Wu, Zhengqing;Lei, Zhensheng;Lei, Zhensheng;Wang, Jiansheng;Huang, Fangfang
关键词：Hexaploid wheat (Triticum aestivum L; ); Ca accumulation; superior alleles; pyramid breeding; genome-wide association analysis
-
Uncovering the genetic basis of gluten aggregation parameters by genome-wide association analysis in wheat (Triticum aestivum L.) using GlutoPeak
作者：Wu, Zhengqing;Qiu, Hongxia;Tian, Zhaoran;Liu, Congcong;Qin, Maomao;Li, Wenxu;Yang, Pan;Tian, Baoming;Wei, Fang;Zhou, Zhengfu;Lei, Zhensheng;Hou, Jinna;Wu, Zhengqing;Qiu, Hongxia;Tian, Zhaoran;Tian, Baoming;Wei, Fang;Zhou, Zhengfu;Lei, Zhensheng;Hou, Jinna;Wen, Yao;Lei, Zhensheng;Zhou, Zhengfu;Lei, Zhensheng
关键词：Wheat; Gluten aggregation properties; GlutoPeak parameters; GWAS
-
Starch Bio-Synthetic Pathway Genes Contribute to Resistant Starch Content Differentiation in Bread Wheat
作者：Hou, Jinna;Deng, Hui;Wang, Yingdong;Liu, Congcong;Geng, Shenghui;Li, Wenxu;Qin, Maomao;Dai, Ziju;Shi, Xia;Yang, Pan;Wu, Zhengqing;Lei, Zhensheng;Zhou, Zhengfu;Hou, Jinna;Tian, Baoming;Wu, Zhengqing;Lei, Zhensheng;Zhou, Zhengfu;Deng, Hui;Yao, Wen;Lei, Zhensheng;Lei, Zhensheng;Zhou, Zhengfu
关键词：resistant starch; genetic loci; KASP marker; nutritional quality; wheat
-
Quantitative traits loci mapping and molecular marker development for total glutenin and glutenin fraction contents in wheat
作者：Zhou, Zhengfu;Chen, Lingzhi;Liu, Congcong;Qin, Maomao;Li, Wenxu;Wu, Zhengqing;Lei, Zhensheng;Hou, Jinna;Zhou, Zhengfu;Tian, Baoming;Wu, Zhengqing;Lei, Zhensheng;Hou, Jinna;Zhang, Ziwei;Chen, Lingzhi;Liu, Congcong;Lei, Zhensheng;Mason, Annaliese S.
关键词：Glutenin content; High- and low-molecular-weight glutenin subunits; End-use quality; QTL mapping; KASP marker; Wheat
