Characterization of Adelphocoris suturalis (Hemiptera: Miridae) Transcriptome from Different Developmental Stages
文献类型： 外文期刊
作者： Tian, Caihong 1 ; Tay, Wee Tek 2 ; Feng, Hongqiang 1 ; Wang, Ying 1 ; Hu, Yongmin 1 ; Li, Guoping 1 ;
作者机构： 1.Henan Acad Agr Sci, Henan Inst Plant Protect, Int Joint Res Lab Crop Protect,Henan Key Lab Crop, Lab Southern Part Northern China,MOA Key Reg Crop, Zhengzhou 450002, Peoples R China
2.CSIRO, Canberra, ACT 2601, Australia
期刊名称：SCIENTIFIC REPORTS （ 影响因子：4.379； 五年影响因子：5.133 ）
ISSN： 2045-2322
年卷期： 2015 年 5 卷
页码：
收录情况： SCI
摘要： Adelphocoris suturalis is one of the most serious pest insects of Bt cotton in China, however its molecular genetics, biochemistry and physiology are poorly understood. We used high throughput sequencing platform to perform de novo transcriptome assembly and gene expression analyses across different developmental stages (eggs, 2nd and 5th instar nymphs, female and male adults). We obtained 20 GB of clean data and revealed 88,614 unigenes, including 23,830 clusters and 64,784 singletons. These unigene sequences were annotated and classified by Gene Ontology, Clusters of Orthologous Groups, and Kyoto Encyclopedia of Genes and Genomes databases. A large number of differentially expressed genes were discovered through pairwise comparisons between these developmental stages. Gene expression profiles were dramatically different between life stage transitions, with some of these most differentially expressed genes being associated with sex difference, metabolism and development. Quantitative real-time PCR results confirm deep-sequencing findings based on relative expression levels of nine randomly selected genes. Furthermore, over 791,390 single nucleotide polymorphisms and 2,682 potential simple sequence repeats were identified. Our study provided comprehensive transcriptional gene expression information for A. suturalis that will form the basis to better understanding of development pathways, hormone biosynthesis, sex differences and wing formation in mirid bugs.
- 相关文献
作者其他论文 更多>>
-
Population Genetics and Trajectory Simulation Reveals the Invasion Process of the Fall Armyworm (Spodoptera frugiperda) in the Eastern Hemisphere
作者：Fu, Pengfei;Sun, Zhongxiang;Chen, Yaping;Lu, Zhihui;Gui, Furong;Li, Xijie;Hu, Gao;Li, Xijie;Tian, Caihong
关键词：Eastern Hemisphere; fall armyworm; genetic evolution; invasion process; migration trajectory
-
The East Asian Insect Flyway Geographical and Climatic Factors Driving Migration Among Diverse Crop Pests
作者：Hu, Gao;Chapman, Jason W.;Feng, Hongqiang;Otuka, Akira;Reynolds, Don R.;Reynolds, Don R.;Drake, Alistair;Drake, Alistair;Chapman, Jason W.;Chapman, Jason W.
关键词：insect migration circuits; population pathways; atmospheric circulation; pest management; moths; planthoppers
-
Can the Generalist Predator Calosoma chinense Kirby Be Effectively Employed in the Biological Control of Spodoptera frugiperda (J. E. Smith)?
作者：Tian, Caihong;Huang, Jianrong;Zhang, Junyi;Li, Guoping;Feng, Hongqiang;Zhang, Junyi;Yin, Xinming;Hao, Xuezheng;Wang, Lin
关键词：
Calosoma chinense Kirby;Spodoptera frugiperda (J. E. Smith); predation capacity; predation choice -
Performance of the Fall Armyworm, Spodoptera frugiperda (Lepidoptera: Noctuidae), over Three Generations on Four Maize Cultivars
作者：Zhang, Bo;Yi, Jing;Yan, Yan;Wang, Yirui;Xue, Yana;Yan, Haiwang;Ren, Meifeng;Li, Daqi;Lu, Junjiao;Li, Guoping
关键词：insect-plant interactions; pest control; agricultural entomology; crop protection; control technology
-
Involvement of Gonolabis distincta in the Control of Root Maggots in Garlic Fields
作者：Tian, Caihong;Li, Junpeng;Zhang, Junyi;Gao, Xinju;Yang, Lirong;Feng, Hongqiang;Zhang, Yan;Zhang, Junyi;Yin, Xinming
关键词：garlic pests; root maggot; DNA barcoding;
Gonolabis distincta ; predation ability -
Can Sweet Maize Act as a Trap Crop for Fall Armyworm?
作者：Tian, Caihong;Zhang, Junyi;Li, Guoping;Huang, Jianrong;Feng, Hongqiang;Zhang, Junyi;Wu, Shaoying;Zhang, Junyi;Yin, Xinming
关键词：
Spodoptera frugiperda ; sweet and normal maize; adult rhythm; circadian clock genes; RT-qPCR -
First high-resolution vertical-looking radar for long-term automatic observation of high-flying insects in Asia
作者：Feng, Hongqiang
关键词：vertical looking radar; long-term monitoring; migratory insect pests; agriculture
