文献类型： 外文期刊

作者： Liu Su ¹ ; Huang Yuan-jie ¹ ; Qiao Fei ¹ ; Zhou Wen-wu ¹ ; Gong Zhong-jun ¹ ; Cheng Jia-an ¹ ; Zhu Zeng-rong ¹ ;

作者机构： 1.Zhejiang Univ, Inst Insect Sci, State Key Lab Rice Biol, Biol Key Lab Agr Entomol,Minist Agr, Hangzhou 310058, Zhejiang, Peoples R China

2.Henan Acad Agr Sci, Inst Plant Protect, Zhengzhou 450002,

关键词： Cnaphalocrocis medinalis;Chilo suppressalis;olfactory co-receptor;molecular cloning;relative expression;transmembrane orientation

期刊名称：JOURNAL OF INTEGRATIVE AGRICULTURE （ 影响因子：2.848； 五年影响因子：2.979 ）

ISSN： 2095-3119

年卷期： 2013 年 12 卷 10 期

页码：

收录情况： SCI

摘要： In insects, the sense of smell is mainly mediated by olfactory receptors (Ors). Olfactory co-receptor (Orco), which is co-expressed with the Ors in almost all olfactory receptor neurons (ORNs), is demonstrated to be an essential component in the insect olfactory system. It can be potential target for developing novel olfactory-disruption strategy to control insect pests. In this study, two full-length cDNA sequences encoding Orcos (CmedOrco and ChsupOrco) were cloned from two Lepidopteran rice pests, the rice leaffolder, Cnaphalocrocis medinalis and the rice striped stem borer, Chilo suppressalis. The amino acid sequences of CmedOrco and ChsupOrco showed high similarity to the previously identified Orcos from other insect species. Bioinformatic prediction and cellular immunofluorescence indicated that CmedOrco and ChsupOrco were both seven-transmembrane proteins with intracellular N-termini and extracellular C-termini. mRNA expression levels of the two Orcos were much higher in male and female antennae than those in non-olfactory tissues, and the ChsupOrco transcripts reached a peak level in adults compared to other life stages. Our results provide a foundation from which it will be possible to elucidate the roles of Orco in moth olfaction and for the development of environment-friendly management strategies of these two rice insect pests.