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Enzymatic preparation of L-alpha-glycerylphosphorylcholine in an aqueous medium

文献类型： 外文期刊

作者： Zhang, Kangyi 1 ; Liu, Yuanfa 1 ; Wang, Xingguo 1 ;

作者机构： 1.Jiangnan Univ, Sch Food Sci & Technol, State Key Lab Food Sci & Technol, Wuxi 214122, Jiangsu, Peoples R China

2.Henan Acad Agr Sci, Inst Agr Prod Proc, Zhengzhou, Henan Province, Peoples R China

关键词： Aqueous medium;L-a-glycerylphosphorylcholine;Phosphatidylcholine;Phospholipase A1 (Lecitase Ultra);Silica gel column chromatography

期刊名称：EUROPEAN JOURNAL OF LIPID SCIENCE AND TECHNOLOGY （ 影响因子：2.679； 五年影响因子：2.627 ）

ISSN： 1438-7697

年卷期： 2012 年 114 卷 11 期

页码：

收录情况： SCI

摘要： L-alpha-Glycerylphosphorylcholine (L-alpha-GPC) was successfully prepared from phosphatidylcholine (PC) of food-grade soy lecithin powder using a novel enzymatic reaction in an aqueous medium. 94.5% yield of L-alpha-GPC was obtained under the optimal conditions of 55 degrees C, 6.67 mg/mL substrate, 2 mM CaCl2, and 33.4 U/mL phospholipase A(1) (Lecitase Ultra). L-alpha-GPC at 98% purity, 73.4% (wt%) recovery, and specific rotation ([alpha](D)(20)) of -2.5 degrees was achieved by silica gel column chromatography. Owing to its excellent catalytic efficiency, low cost, and ready availability, phospholipase A(1) (Lecitase Ultra) provides a very satisfactory option for converting PC to L-alpha-GPC. Practical applications: L-alpha-Glycerylphosphorylcholine (L-alpha-GPC) has been studied recently for its potential use as a supplement that may support neurological functions, but it is only found in trace amounts in nature. The present results indicate that Lecitase Ultra can be used for producing L-alpha-GPC from aqueous PC and suggest encouraging prospects for practical or industrial applications utilizing its notable catalytic performance, economy, and convenience.

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