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Possible involvement of maize Rop1 in the defence responses of plants to viral infection

文献类型: 外文期刊

作者: Cao, Yanyong 1 ; Shi, Yan 1 ; Li, Yongqiang 1 ; Cheng, Yuqin 1 ; Zhou, Tao 1 ; Fan, Zaifeng 1 ;

作者机构: 1.China Agr Univ, State Key Lab Agrobiotechnol, Beijing 100193, Peoples R China

2.China Agr Univ, Dept Plant Pathol, Beijing 100193, Peoples R China

3.Henan Acad Agr Sci, Cereal Crops Inst, Zhengzhou 450002, Peoples R China

期刊名称:MOLECULAR PLANT PATHOLOGY ( 影响因子:5.663; 五年影响因子:5.626 )

ISSN: 1464-6722

年卷期: 2012 年 13 卷 7 期

页码:

收录情况: SCI

摘要: The expression of host genes can be altered during the process of viral infection. To investigate the viral infection-induced up-regulated gene expression changes of maize at different time intervals post-inoculation with Sugarcane mosaic virus (SCMV), a suppression subtractive hybridization cDNA library was constructed. A total of 454 cDNA clones were identified to be viral infection-induced up-regulated genes. The influence of Rop1 on the infection of maize by SCMV was investigated. The results showed that transient silencing of the ZmRop1 gene through virus-induced gene silencing enhanced the accumulation and systemic infection of SCMV and another potyvirus (Pennisetum mosaic virus) in maize plants, whereas transient over-expression of ZmRop1 in maize protoplasts reduced SCMV accumulation. Furthermore, it was demonstrated that the heterologous expression of ZmRop1 impaired Potato virus X infection in Nicotiana benthamiana plants. These data suggest that ZmRop1 may play a role in plant defence responses to viral infection.

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