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Improvement of the photosynthetic characteristics of transgenic wheat plants by transformation with the maize C4 phosphoenolpyruvate carboxylase gene

文献类型： 外文期刊

作者： Hu, Lin 1 ; Li, Yan 1 ; Xu, Weigang 1 ; Zhang, Qingchen 1 ; Zhang, Lei 1 ; Qi, Xueli 1 ; Dong, Haibin 1 ;

作者机构： 1.Henan Acad Agr Sci, Wheat Res Ctr, Zhengzhou 450002, Henan, Peoples R China

2.Nanjing Agr Univ, Natl Key Lab Crop Genet & Germplasm Enhancement, Nanjing 210095, Jiangsu, Peoples R China

关键词： gene cloning;photosynthesis;transgenic wheat;Zea mays phosphoenolpyruvate carboxylase

期刊名称：PLANT BREEDING （ 影响因子：1.832； 五年影响因子：1.956 ）

ISSN： 0179-9541

年卷期： 2012 年 131 卷 3 期

页码：

收录情况： SCI

摘要： With 7 figures and 2 tables Abstract Attempts have been made to transform C3 crops with C4 genes to improve the photosynthetic rate of C3 plants. In our study, a full-length cDNA for phosphoenolpyruvate carboxylase gene (pepc) was isolated from Zea mays by PCR. The pepc gene was introduced into wheat plants (Triticum aestivum) by particle bombardment transformation. The integration, transcription and expression of the pepc gene were confirmed by Southern blot, RT-PCR and Western blot analysis. Physiological studies showed that phosphoenolpyruvate carboxylase activity in the transgenic plants was 140% higher than that of the untransformed plants. The highest rate of photosynthesis in the transgenic plants was 31.95 mu mol CO2/m2/s, which was 26% greater than the rate in untransformed plants. The light saturation point and carboxylation efficiency of pepc transgenic wheat were 20% and 22.57% higher, respectively, than those of untransformed wheat. As a result, the weight of seed per spike and thousand-grain weight were 0.23 g and 1.21 g higher than those of untransformed wheat. Our research will facilitate the further study of the function of the pepc gene in wheat plants.

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