您好,欢迎访问河南省农业科学院 机构知识库!

Verification and fine-mapping of QTLs conferring days to flowering in soybean using residual heterozygous lines

文献类型: 外文期刊

作者: Su ChengFu 1 ; Lu WeiGuo 1 ; Zhao TuanJie 1 ; Gai JunYi 1 ;

作者机构: 1.Nanjing Agr Univ, Soybean Res Inst, Nanjing 210095, Peoples R China

2.Natl Ctr Soybean Improvement, Nanjing 210095, Peoples R China

3.Natl Key Lab Crop Genet & Germplasm Enhancement, Nanjing 210095, Peoples R China

4.Henan Acad Agr Sci, Inst Cotton & Oil Crops, Zhengzhou 450002, Peoples R China

关键词: soybean;backcross line;RHL (residual heterozygous line);SSR (simple sequence repeat) marker;flowering date;QTL;fine-mapping

期刊名称:CHINESE SCIENCE BULLETIN ( 2016影响因子:1.649; 五年影响因子:1.738 )

ISSN: 1001-6538

年卷期: 2010 年 55 卷 6 期

页码:

收录情况: SCI

摘要: The results of QTL mapping based on a primary mapping population should be further verified and refined for its real utilization in marker-assisted selection or map-based cloning. The primary mapping population contains 114 BC(1)F(1) plants of the backcross between Essex (maturity group V, MG V) as the donor parent and ZDD2315 (MG II) as the recurrent parent. In this study, a genetic linkage map with 250 SSR markers spanning a total length of 2963.5 cM on 25 linkage groups (LG) was constructed using software MAPMAKER3.0. Six kinds of genetic statistical models of 4 softwares, i.e. WinQTL Cartographer Version 2.5, IciMapping Version 2.0, MapQTL Version 5.0 and QTLnetwork Version 2.0, were used to map QTLs conferring days to flowering of the BC(1)F(3) lines. Nine QTLs were mapped on 6 different linkage groups (LG). Of those, 6 QTLs were detected by at least two different genetic statistical models, while the other three were detected by only one procedure. Among the three QTLs, Flwdt7 was mapped between Sat_213 and Satt643 on LG C2 with only 11.0% contribution rate. For confirmation of Flwdt7, 5 RHL populations were developed through selfing eight BC(1)F(5) plants heterozygous at seven markers around the locus. The RHL populations with the same segregating loci were bulked and used to construct a secondary linkage map of the specific segment using software JoinMapA (R) 3.0. The genetic distances among the markers on the specific segment became shorter than those of the whole genome map. On the secondary map, Flwdt7 was mapped between Satt277 and Satt489, next to its primary interval Sat_213-Satt643, with distance 1.40 cM to Satt277 and 0.45 cM to Satt489, confidence interval narrowed to 2.7 cM, and contribution rate increased to 36.8%. The results were confirmed with significance analysis among marker genotypes on individual loci and comparison analysis of target marker intervals among near isogenic lines (plants). Thus the strategy by using residual heterozygous lines for QTL fine-mapping on target segments based on primary whole genome scanning with multiple mapping models was demonstrated to be effective.

  • 相关文献

[1]QTL Mapping of Isoflavone, Oil and Protein Contents in Soybean (Glycine max L. Merr.). Liang Hui-zhen,Yu Yong-liang,Wang Shu-feng,Lian Yun,Wang Ting-feng,Wei Yan-li,Gong Peng-tao,Fang Xuan-jun,Liu Xue-yi,Zhang Meng-chen. 2010

[2]Identification and Fine Mapping of rhm1 Locus for Resistance to Southern Corn Leaf Blight in Maize. Zhao, Yuanzeng,Lu, Xiaomin,Liu, Chaoxian,Guan, Haiying,Zhang, Mei,Li, Zhongfeng,Cai, Hongwei,Lai, Jinsheng,Zhao, Yuanzeng,Lu, Xiaomin,Liu, Chaoxian,Guan, Haiying,Zhang, Mei,Li, Zhongfeng,Cai, Hongwei,Lai, Jinsheng,Zhao, Yuanzeng,Lu, Xiaomin. 2012

[3]Identification of QTLs involved in pod-shatter resistance in Brassica napus L.. Wen, Y. C.,Zhang, S. F.,Wang, J. P.,Zhu, J. C.,He, J. P.,Cao, J. H.,Yi, B.,Wen, J..

[4]Epistatic and QTLxenvironment interaction effects on leaf area-associated traits in maize. Wei, Xiaomin,Wang, Xiaobo,Zhou, Jinlong,Shi, Yong,Wang, Huitao,Dou, Dandan,Song, Xiaoheng,Li, Guohui,Ku, Lixia,Chen, Yanhui,Wei, Xiaomin,Wang, Xiaobo,Zhou, Jinlong,Shi, Yong,Wang, Huitao,Dou, Dandan,Song, Xiaoheng,Li, Guohui,Ku, Lixia,Chen, Yanhui,Wei, Xiaomin,Guo, Shulei.

[5]Genetic dissection of seed vigour under artificial ageing conditions using two joined maize recombinant inbred line populations. Ku, Lixia,Cui, Xinjian,Guo, Shulei,Tian, Zhiqiang,Han, Tuo,Ren, Zhenzhen,Zhang, Liangkun,Su, Huihui,Chen, Yanhui,Cheng, Fangfang,Qi, Jianshuang.

[6]大豆品质性状QTL定位的研究进展. 位艳丽,余永亮,练云,王树峰,王庭峰,梁慧珍. 2010

[7]6种大豆粒形性状的QTL定位. 梁慧珍,王树峰,余永亮,王庭峰,巩鹏涛,方宣钧,刘学义,赵双进,张孟臣,李卫东. 2008

[8]谷子萌芽期抗旱相关QTL定位研究. 代小冬,朱灿灿,王春义,秦娜,宋迎辉,代书桃,李君霞. 2020

[9]玉米穗三叶叶宽QTL定位及Meta分析. 张莹莹,杨青,代资举,王艳,王新涛. 2019

[10]谷子苗期耐低氮相关性状的QTL分析. 秦娜,付森杰,朱灿灿,代书桃,宋迎辉,魏昕,王春义,叶珍言,李君霞. 2023

[11]玉米不同叶位叶面积的QTL定位. 安允权,张君,席章营,李明娜,李沛. 2016

[12]大豆抗胞囊线虫(Heterodera glycines Ichinohe)基因定位研究进展. 卢为国,李卫东,梁惠珍,王树峰,孙慎. 2005

[13]大豆α-生育酚的遗传与QTL分析. 梁慧珍,余永亮,许兰杰,杨红旗,董薇,谭政伟,李磊,裴新涌,刘新梅. 2019

[14]花生主要品质性状的QTLs定位分析. 张新友,韩锁义,徐静,严玫,刘华,汤丰收,董文召,黄冰艳. 2012

[15]不同氮磷钾处理大豆苗期主根长和侧根数的QTL定位分析. 梁慧珍,董薇,许兰杰,余永亮,杨红旗,谭政伟,许阳,陈鑫伟. 2017

[16]基于玉米重组自交系籽粒脱水速率的相关性状分析. 王延召,魏良明,周波,宋迎辉. 2016

[17]玉米穗三叶叶长和叶面积的QTL定位. 王新涛,杨青,李保叶,代资举,郝俊杰. 2022

[18]不同营养水平水培条件下幼苗期大豆下胚轴性状QTL定位与上位互作分析. 梁慧珍,董薇,许兰杰,余永亮,杨红旗,谭政伟,夏伟,陈鑫伟. 2018

[19]大豆异黄酮与脂肪、蛋白质含量基因定位分析. 梁慧珍,王树峰,余永亮,练云,王庭峰,位艳丽,巩鹏涛,刘学义,方宣钧. 2009

[20]幼苗期大豆根系性状的遗传分析与QTL检测. 梁慧珍,余永亮,杨红旗,张海洋,董薇,崔暐文,巩鹏涛,方宣钧. 2014

作者其他论文 更多>>