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Development of a lateral-flow immunochromatographic test device for the rapid detection of difloxacin residues

文献类型： 外文期刊

作者： Zhi, Ai-min 1 ; Li, Bin-bin 1 ; Liu, Qing-tang 1 ; Hu, Xiao-fei 1 ; Zhao, Dong 1 ; Hou, Yu-ze 1 ; Deng, Rui-guang 1 ; Cha 1 ;

作者机构： 1.Henan Acad Agr Sci, Key Lab Anim Immunol, Zhengzhou 450002, Peoples R China

2.Jiaozuo Entry Exit Inspect & Quarant Bur PR China, Henan 473036, Peoples R China

关键词： difloxacin;test devices;colloidal gold;immunochromatography;rapid test;immunoassays

期刊名称：FOOD AND AGRICULTURAL IMMUNOLOGY （ 影响因子：3.101； 五年影响因子：2.573 ）

ISSN： 0954-0105

年卷期： 2010 年 21 卷 4 期

页码：

收录情况： SCI

摘要： In this study, a rapid competitive immunochromatographic test strip has been developed for specifically testing residues of difloxacin (DIF). 1H10-2B2, one of the three hybridoma lines that were tested and selected by enzyme linked immunosorbent assay, was used in the test strip. The monoclonal antibody has a good sensitivity with an IC50 of 2.2 ng/mL to DIF, 0.24% cross-reactivity towards danofloxacin and no cross-reactivity towards other related compounds and other drugs. The calibration curve of DIF test strip presented a typical sigmoidal curve. In practice, the lower limit of detection using a strip reader was 0.5 ng/ml while 2 ng/ml by unaided visual assessment. Samples of milk were spiked at 2-8 ng/mL, presenting recoveries between 94.5 and 107%, and the coefficient of variation (CV,%) (1.6-9%). The data above suggests that the strip meets the requirements of high sensitivity, good specificity, simplicity and speed, as well as the characteristics of reproducibility and accuracy.

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