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Seed storage protein components are associated with curled cotyledon phenotype in soybean

文献类型： 外文期刊

作者： Han, Suoyi 1 ; Fan, Rui 1 ; Zhao, Tuanjie 1 ; Yu, Jingjing 1 ; Yu, Deyue 1 ;

作者机构： 1.Nanjing Agr Univ, Natl Ctr Soybean Improvement, Nanjing 210095, Peoples R China

2.Henan Acad Agr Sci, Econ Crops Res Inst, Zhengzhou 450002, Peoples R China

关键词： Soybean;cotyledon mutant;seed storage protein;glycinin subunits;lectin

期刊名称：AFRICAN JOURNAL OF BIOTECHNOLOGY （ 影响因子：0.573； 五年影响因子：0.794 ）

ISSN： 1684-5315

年卷期： 2009 年 8 卷 22 期

页码：

收录情况： SCI

摘要： Local soybean cultivar "Nannong 94-16" was treated with NaN3-Co-60 gamma ray and EMS separately, and two curled cotyledon mutants were obtained. Local soybean cultivar "TSB" was treated with Co-60 gamma ray and one curled cotyledon mutant was obtained. Two-dimension electrophoresis analysis was employed to determine the differential proteins between three mutants and their wild types. The results showed that there were three common differential protein spots between the mutants and their wild types. Further analysis suggests that the two increased protein spots in mutants were both globulin subunit G3/A1aB1b, while the decreased protein spot in mutants was lectin. These results indicate that there may be an association between protein components (glycinin subunits G3/A1aB1b, lectin) and the curled cotyledon phenotype in soybean.

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