Genomic sequencing and characterization of a Chinese isolate of Bovine viral diarrhea virus 2
文献类型： 外文期刊
作者： Zhu, L. Q. 1 ; Lin, Y. Q. 1 ; Ding, X. Y. 2 ; Ren, M. 1 ; Tao, J. 1 ; Wang, J. Y. 1 ; Zhang, G. P. 3 ; Zhu, G. Q. 1 ;
作者机构： 1.Yangzhou Univ, Coll Vet Med, Yangzhou 225009, Peoples R China
2.NW A&F Univ, Coll Life Sci, Yangling 712100, Peoples R China
3.Henan Acad Agr Sci, Minist Agr, Key Lab Anim Immunol, Zhengzhou 450002, Peoples R China
关键词： BVDV-2;cytopathic effect;genomic sequencing;phylogenetic analysis
期刊名称：ACTA VIROLOGICA （ 影响因子：1.162； 五年影响因子：1.255 ）
ISSN： 0001-723X
年卷期： 2009 年 53 卷 3 期
页码：
收录情况： SCI
摘要： The complete genomic sequencing and characterization of Bovine viral diarrhea virus (BVDV) isolate XJ-04 originated from cattle in China was described. The genomic RNA of the isolate was 12,284 nt long and contained short 5'- untranslated region (UTR), Y-non-coding regions (NCR), and one open reading frame (ORF) encoding a large polyprotein of 3,895 amino acids with 20 potential N-glycosylation sites. The identity of the isolate XJ-04 with reference strains NADL (BVDV-1) and 890 (BVDV-2) in autoproteasc (N(pro)) gene and structural genes (C, E(rns), El, E2) was analyzed. The percentage of nt and aa identity in analyzed genes indicated that the isolate XJ-04 was closer to the reference strain 890 (BVDV-2) than to NADL (BVDV-1). Phylogenetic analysis revealed that the isolate belonged to BVDV-2a subtype. Furthermore, comparison analysis indicated that the isolate XJ-04 did not contain any genomic insertions that can be directly related to the cytopathic phenotype.
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