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Identification and preliminary analysis of a new PCP promoter from Brassica rapa ssp chinensis

文献类型： 外文期刊

作者： Zhang, Qiang 1 ; Liu, Huizhi 3 ; Cao, Jiashu 1 ;

作者机构： 1.Zhejiang Univ, Inst Vegetable Sci, Lab Cell & Mol Biol, Hangzhou 310029, Zhejiang, Peoples R China

2.Henan Acad Agr Sci, Inst Hort, Zhengzhou 450002, Peoples R China

3.Zhejiang Univ, Inst Biol Sci, Hangzhou 310029, Zhejiang, Peoples R China

关键词： Brassica rapa ssp Chinensis;Brassica campestris Male Fertile 5(BcMF5);PCP;Promoter

期刊名称：MOLECULAR BIOLOGY REPORTS （ 影响因子：2.316； 五年影响因子：2.357 ）

ISSN： 0301-4851

年卷期： 2008 年 35 卷 4 期

页码：

收录情况： SCI

摘要： The promoter of Brassica campestris Male Fertile 5 (BcMF5), a pollen coat protein member, class A (PCP-A) gene family, was isolated from Brassica rapa L. ssp. chinensis Makino (Chinese cabbage-pak-choi) by Thermal Asymmetric Interlaced Polymerase Chain Reaction (TAIL-PCR). Sequence analysis suggested that the 605-bp promoter of BcMF5 appears to be a pollen promoter. In an attempt to confirm the promoter activity of BcMF5 promoter, -609 to +3 bp and -377 to +3 bp fragments of the upstream sequence of BcMF5 were inserted at the site upstream of the coding region of the uidA gene in the sense orientation to construct two deletion expression vectors. Transient expression analysis in onion epidermal cells by particle bombardment showed that both -609 to +3 bp and -377 to +3 bp fragments of BcMF5 promoter were capable of driving beta-glucuronidase gene expression. Furthermore, by Agrobacterium-mediated genetic transformation method, Arabidopsis transgenic Kan(R) plants were obtained. GUS assay analysis revealed that the promoter of BcMF5 induced gene expression at the early stage of anther development and drove high levels of GUS expression in anther walls, upper regions of petals, pollen, and pollen tubes in the middle and late stage of anther development, but did not drive any expression in sepals and pistils.

  • 相关文献

[1]Characterization and functional analysis of a novel PCP gene BcMF5 from Chinese cabbage (Brassica campestris L. ssp chinensis Makino). Cao, Jiashu,Huang, Li,Xiang, Xun,Yu, Xiaolin,Zhang, Qiang,Liu, Huizhi. 2008

[2]BcMF5, a pollen coat protein gene (PCP), from Brassica rapa. ssp chinensis, involved in the transcription of different lengths of 3 '-UTRs of PCPs. Zhang, Qiang,Cao, Jiashu,Huang, Li,Xiang, Xun,Yu, Xiaolin,Zhang, Qiang. 2008

[3]Expression pattern of diacylglycerol acyltransferase-1, an enzyme involved in triacylglycerol biosynthesis, in Arabidopsis thaliana. Lu, CFL,de Noyer, SB,Hobbs, DH,Kang, JL,Wen, YC,Krachtus, D,Hills, MJ.

[4]Characterization of a Maize Wip1 Promoter in Transgenic Plants. Zhang, Shengxue,Liu, Yan,Wang, Xiaoqing,Liu, Yunjun,Wang, Guoying,Lian, Yun. 2013

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