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Development of molecular markers linked to the wheat powdery mildew resistance gene Pm4b and marker validation for molecular breeding

文献类型: 外文期刊

作者: Yi, Y. J. 1 ; Li, H. Y. 1 ; Huang, X. Q. 3 ; An, L. Z. 2 ; Wang, F. 1 ; Wang, X. L. 2 ;

作者机构: 1.Henan Acad Agr Sci, Inst Plant Protect, Zhengzhou 450002, Peoples R China

2.Lanzhou Univ, Minist Educ, Key Lab Arid & Grassland Ecol, Lanzhou 730000, Peoples R China

3.Univ Manitoba, Dept Plant Sci, Winnipeg, MB R3T 2N2, Canada

关键词: Triticum aestivum;Pm4b;SRAP;STS;SSR;marker-assisted selection

期刊名称:PLANT BREEDING ( 影响因子:1.832; 五年影响因子:1.956 )

ISSN: 0179-9541

年卷期: 2008 年 127 卷 2 期

页码:

收录情况: SCI

摘要: Powdery mildew, caused by Blumeria graminis (DC.) E.O. Speer f. sp. tritici, is an important disease in wheat (Triticum aestivum L.). Bulk segregant analysis (BSA) was employed to identify SRAP (sequence-related amplified polymorphism), sequence tagged site (STS) and simple sequence repeat (SSR) markers linked to the Pm4b gene, which confers good resistance to powdery mildew in wheat. Out of 240 SRAP primer combinations tested, primer combinations Me8/Em7 and Me12/Em7 yielded 220-bp and 205-bp band, respectively, each of them associated with Pm4b. STS-(241) also linked to Pm4b with a genetic distance of 4.9 cM. Among the eight SSR markers located on wheat chromosome 2AL, Xgwm382 was found to be polymorphic and linked to Pm4b with a genetic distance of 11.8 cM. Further analysis was carried out using the four markers to investigate marker validation for marker-assisted selection (MAS). The results showed that a combination of the linked markers STS-(241), Me8/Em7-(220) and Xgwm382 could be used for marker-assisted selection of the resistance gene Pm4b in wheat breeding programmes.

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