文献类型： 外文期刊

作者： Wang, XW ¹ ; Lia, JR ² ; Chen, LH ² ; Liu, GT ² ;

作者机构： 1.Chinese Acad Sci, Inst Genet, Beijing 100101, Peoples R China

2.Chinese Acad Sci, Inst Genet, Beijing 100101, Peoples R China; China Agr Univ, Dept Genet & Plant Breeding, Beijing 100094, Peoples R China; Henan Acad Agr Sci, Wheat Inst, Zhengzhou 450002, Peoples R China

关键词： common wheat;Chinese Spring;ph1b mutant;sequence characterized amplified region;(SCAR) marker;marker-assistant selection

期刊名称：ACTA BOTANICA SINICA （ 影响因子：0.599； ）

ISSN： 0577-7496

年卷期： 2000 年 42 卷 3 期

页码：

收录情况： SCI

摘要： The genomic DNA of common wheat (Triticum aestivum L.) "Chinese Spring" (CS) and its ph1b mutant were analyzed by using 19 sequence tagged site PCR (STS-PCR) primers, which derived from RFLP probes from barley ( Hordeum vulgare L.) chromosome 5H. One marker was identified on wheat chromosome 5BL, which is 5.7 cM (centiMorgan) proximal to Ph1 gene, using the CS homoeologous group 5 nullisomic-tetrasomic, ditelosomic 5BL line and an F-2 population from CS x ph1b mutant. This linked PCR marker was converted into a more specific sequence characterized amplified region (SCAR) marker. To obtain a new winter wheat line containing phlb gene, the authors used a nullisomic 5B line of "Abbodanza" as a bridge parent and crossed respectively with the CS ph1b mutant (donor) and a winter wheat variety, "Jing 411" (recipient). The meiotic chromosome pairing was checked in the progeny of each cross, as well as using the marker-assistant selection of the SCAR marker identified for phlb gene.,After three inter-crossing and one selfing, a relatively stable ph1b substitution line of winter wheat with "Jing 411" background way obtained.