Determination of gamma-hydroxybutyrate in human urine samples by ion exclusion and ion exchange two-dimensional chromatography system
文献类型： 外文期刊
作者： Liu, Junwei 1 ; Deng, Zhifen 2 ; Zhu, Zuoyi 3 ; Wang, Yong 4 ; Wang, Guoqing 1 ; Sun, Yu-an 1 ; Zhu, Yan 5 ;
作者机构： 1.Zhengzhou Univ Light Ind, Dept Appl Chem, Zhengzhou 450002, Henan, Peoples R China
2.Zhengzhou Univ, Coll Chem & Mol Engn, Zhengzhou 450001, Henan, Peoples R China
3.Zhejiang Acad Agr Sci, Inst Qual & Stand Agr Prod, Hangzhou 310021, Zhejiang, Peoples R China
4.Nanjing Publ Secur Bur, Inst Forens Sci & Technol, Nanjing 210001, Jiangsu, Peoples R China
5.Zhejiang Univ, Dept Chem, Xixi Campus, Hangzhou 310028, Zhejiang, Peoples R China
关键词： gamma-hydroxybutyrate;Two-dimensional chromatography;Ion exclusion;Ion exchange;Column switching
期刊名称：JOURNAL OF CHROMATOGRAPHY A （ 影响因子：4.759； 五年影响因子：4.302 ）
ISSN： 0021-9673
年卷期： 2017 年 1528 卷
页码：
收录情况： SCI
摘要： A two-dimensional ion chromatography system was developed for the determination of gamma-hydroxybutyrate (GHB) in human urine samples. Ion exclusion chromatography was used in the first dimensional separation for elimination of urine matrices and detection of GHB above 10 mg L-1, ion exchange chromatography was used in the second dimensional separation via column-switching technique for detection of GHB above 0.08 mgL(-1). Under the optimized chromatographic conditions, the ion exclusion and ion exchange chromatography separation system exhibited satisfactory repeatability (RSD < 3.01%, n=6) and good linearity in the range of 50-1000 mg L-1 and 0.5-100 mg L-1, respectively. By this method, concentrations of GHB in the selected human urine samples were detected in the range of 0-1.57 mg L-1 . The urine sample containing 0.89 mg L-1 GHB was selected to evaluate the accuracy; the spiked recoveries of GHB were 95.9-102.8%. The results showed that the two-dimensional ion chromatography system was convenient and practical for the determination of GHB in human urine samples. (C) 2017 Elsevier B.V. All rights reserved.
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