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Overexpression of the mulberry latex gene MaMLX-Q1 enhances defense against Plutella xylostella in Arabidopsis thaliana

文献类型： 外文期刊

作者： Liu, Yan 1 ; Ji, Dongfeng 1 ; Chen, Jine 1 ; Lin, Tianbao 1 ; Wei, Jia 1 ; Zhu, Yan 1 ; Lv, Zhiqiang 1 ;

作者机构： 1.Zhejiang Acad Agr Sci, Sericultural Res Inst, Hangzhou 310021, Zhejiang, Peoples R China

关键词： chitinase;Plutella xylostella;transgenic Arabidopsis thaliana;latex;mulberry

期刊名称：ARCHIVES OF BIOLOGICAL SCIENCES （ 影响因子：0.956； 五年影响因子：0.943 ）

ISSN： 0354-4664

年卷期： 2017 年 69 卷 2 期

页码：

收录情况： SCI

摘要： Purified mulberry latex chitinase (MLX) has a role in defense against some lepidopteran insects. In this study, a full length chitinase gene, MaMLX-Q1, of 1405 bp with a 1140 bp open reading frame, was obtained from mulberry leaves by the degenerate primers and rapid amplification of cDNA ends polymerase chain reaction (RACE-PCR) procedure. The gene encoded a mature protein with the predicted molecular mass of 39.38 kDa and an isoelectric point (pI) of 6.43; it contained two chitin-binding domains and a hydrolase family 19 chitinase domain. Sequence alignment and phylogenetic analysis grouped it in the class I chitinase protein group. Heterogeneous expression of this MaMLX-Q1 in Arabidopsis showed non-visible alterations in growth phenotype, except for the higher transcriptional expression of MaMLX-Q1 when compared to that of wild-type Arabidopsis. This ectopic MaMLX-Q1 exhibited toxicity to the growth and development of Plutella xylostella larvae, causing significantly lower weight gain and higher mortality. These results indicate an application of MaMLX-Q1 as an insecticide for plant protection.

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