OspTAC2 encodes a pentatricopeptide repeat protein and regulates rice chloroplast development
文献类型: 外文期刊
作者: Wang, Dekai 1 ; Liu, Heqin 1 ; Zhai, Guowei 1 ; Wang, Liangsheng; Shao, Jianfeng 1 ; Tao, Yuezhi 1 ;
作者机构: 1.Zhejiang Acad Agr Sci, Inst Crop & Nucl Technol Utilizat, Hangzhou 310021, Zhejiang, Peoples R China
2.Zhejiang Acad Agr Sci, State Key Lab Breeding Base Zhejiang Sustainable, Hangzhou 310021, Zhejiang, Peoples R China
3.Zhejiang Acad Agr Sci, State Key Lab Breeding
关键词: Oryza sativa;Pentatricopeptide repeat protein;Chloroplast development
期刊名称:JOURNAL OF GENETICS AND GENOMICS ( 影响因子:4.275; 五年影响因子:5.223 )
ISSN: 1673-8527
年卷期: 2016 年 43 卷 10 期
页码:
收录情况: SCI
摘要: Functional chloroplast generation depends on the precise coordination of gene expression between the plastid and the nucleus and is essential for plant growth and development. In this study, a rice (Oryza sativa) mutant that exhibited albino and seedling-lethal phenotypes was isolated from a60Co-irradiated rice population. The mutant gene was identified as an ortholog of the Arabidopsis plastid transcriptionally active chromosome protein 2 (pTAC2) gene, and the mutant strain was designated osptac2. Sequence and transcription analyses showed that OspTAC2 encodes a putative chloroplast protein consisting of 10 pentratricopeptide repeat (PPR) domains and a C-terminal small MutS-related (SMR) domain. Cytological observations via microscopy showed that the OspTAC2-green fluorescent fusion protein is localized in the chloroplasts. Transmission electron microscopy revealed that the chloroplast of the osptac2 mutant lacks an organized thylakoid membrane. The transcript levels of all investigated PEP (plastid-encoded RNA polymerase)-dependent genes were dramatically reduced in the osptac2 mutant, whereas the transcript levels of NEP (nuclear-encoded polymerase)-dependent genes were increased. These results suggest that OspTAC2 plays a critical role in chloroplast development and indicate that the molecular function of the OspTAC2 gene is conserved in rice and Arabidopsis. Copyright (C) 2016, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, and Genetics Society of China. Published by Elsevier Limited and Science Press. All rights reserved.
- 相关文献
作者其他论文 更多>>
-
GWAS of grain color and tannin content in Chinese sorghum based on whole-genome sequencing
作者:Zhang, Liyi;Xu, Jianxia;Ding, Yanqing;Cao, Ning;Gao, Xu;Feng, Zhou;Cheng, Bing;Zhou, Lengbo;Tao, Yuezhi;Zou, Guihua;Feng, Zhou;Li, Kuiying;Ren, Mingjian
关键词:
-
Effects of Heat Stress during Seed Filling Stage on Brassica napus Seed Oil Accumulation and Chlorophyll Fluorescence Characteristics
作者:Huang, Ruizhi;Yang, Yong;Wu, Xuelong;Liu, Zhihong;He, Haiyan;Wu, Gengwei;Wang, Wengjia;Wang, Hua;Yu, Huasheng;Liu, Heqin
关键词:Brassica napus L.; heat stress; seed filling stage; oil accumulation; chlorophyll fluorescence characteristics
-
Selection Signatures in Chinese Sorghum Reveals Its Unique Liquor-Making Properties
作者:Zhang, Liyi;Ding, Yanqing;Xu, Jianxia;Gao, Xu;Cao, Ning;Feng, Zhou;Cheng, Bing;Zhou, Lengbo;Li, Kuiying;Feng, Zhou;Ren, Mingjian;Lu, Xiaochun;Bao, Zhigui;Tao, Yuezhi;Zou, Guihua;Xin, Zhanguo
关键词:sorghum; selective sweep; population structure; genome-wide association study (GWAS); liquor-brewing related traits
-
Metabolomics Reveals Nutritional Diversity among Six Coarse Cereals and Antioxidant Activity Analysis of Grain Sorghum and Sweet Sorghum
作者:Zhao, Yao;Zhai, Guowei;Tao, Han;Li, Linying;He, Yuqing;Zhang, Xueying;Wang, Fulin;Hong, Gaojie;Zhu, Ying;Zhao, Yao;Tao, Han;Li, Linying;He, Yuqing;Zhang, Xueying;Hong, Gaojie;Li, Xuetong
关键词:coarse cereals; widely targeted metabolome analysis; grain sorghum; sweet sorghum; flavonoids; antioxidant activity
-
The LARGE2-APO1/APO2 regulatory module controls panicle size and grain number in rice
作者:Huang, Luojiang;Xu, Ran;Zhang, Guozheng;Duan, Penggen;Zhang, Baolan;Liu, Zupei;Li, Na;Li, Yunhai;Huang, Luojiang;Hua, Kai;Zhang, Guozheng;Liu, Zupei;Yao, Shanguo;Li, Yunhai;Hua, Kai;Wang, Ruci;Yao, Shanguo;Zeng, Dali;Dong, Guojun;Lu, Xueli;Qian, Qian;Fang, Na;Luo, Yuehua;Wang, Dekai;Yao, Shanguo;Li, Yunhai
关键词:
-
Integrating transcriptome and metabolome reveals molecular networks involved in genetic and environmental variation in tobacco
作者:Liu, Pingping;Zheng, Qingxia;Chen, Qiansi;Zhai, Niu;Xu, Yalong;Jin, Lifeng;Xu, Guoyun;Cao, Peijian;Zhou, Huina;Luo, Jie;Xu, Shengchun;Wang, Gangjun;Shao, Jianfeng;Lu, Xin;Xu, Guowang;Xu, Hai-Ming;Xu, Hai-Ming;Wang, Xusheng
关键词:tobacco; omics integration; gene regulatory networks; co-expression module; molecular network
-
Sorghum qTGW1a encodes a G-protein subunit and acts as a negative regulator of grain size
作者:Zou, Guihua;Zhai, Guowei;Li, Sujuan;Liu, Heqin;Tao, Yuezhi;Yan, Song;Zhou, Lengbo;Ding, Yanqing;Zhang, Liyi;Zhang, Zhipeng;Zou, Jianqiu;Chen, Junping;Xin, Zhanguo
关键词:Cloning; fine mapping; grain size; overexpression; qTGW1a; sorghum