文献类型： 外文期刊

作者： Yang, Yan-qi ¹ ; Liang, Yu-jian ¹ ; Zhi, Jun-rui ¹ ; Li, Ding-yin ¹ ; Li, Cao ¹ ;

作者机构： 1.Guizhou Univ, Inst Entomol, Guizhou Prov Key Lab Agr Pest Management Mountaino, Guiyang 550025, Peoples R China

2.Guizhou Acad Agr Sci, Inst Plant Protect, Guiyang, Peoples R China

关键词： chitin synthase; fall armyworm; glucose content; Lepidoptera; mortality; Noctuidae; pest control; RT-qPCR; trehalase; trehalase inhibitors; trehalose

期刊名称：ENTOMOLOGIA EXPERIMENTALIS ET APPLICATA （ 影响因子：1.4； 五年影响因子：2.1 ）

ISSN： 0013-8703

年卷期： 2024 年

页码：

收录情况： SCI

摘要： Trehalase (TRE) is a key enzyme for degrading trehalose, which plays a vital role in the growth and development of insects. Although validamycin, a compound belonging to a class of efficient antibiotics and fungicides, can control pests by suppressing TRE activities, it remains unknown whether it acts on both trehalose and chitin metabolism in Spodoptera frugiperda (J. E. Smith) (Lepidoptera: Noctuidae), a major pest of maize (Zea mays L., Poaceae). This study investigated the changes in trehalose metabolism after validamycin treatment in S. frugiperda and its effects on the downstream chitin synthesis pathway. Compared with the control, S. frugiperda exhibited varying degrees of mortality after treatment with four concentrations of validamycin, showing a dose-dependent increase in mortality rate. The mortality rates 24 and 48 h after treatment with 0.07 mg mu L-1 validamycin were 35.6% and 42.2%, respectively, indicating the effective lethal concentration. Treatment with 0.07 mg mu L-1 validamycin led to developmental delay, abnormal molting, and death in S. frugiperda, but it exerted no lasting effects on the survival rate, pupal weight, and phenotype during its subsequent developmental stages. At 24 h after validamycin treatment, TRE1 and TRE2 activities and glucose content decreased significantly, whereas the trehalose content increased significantly. Treatment with validamycin significantly upregulated TRE1 and TRE2 expression after 24 and 48 h and downregulated the mRNA expression of chitin synthase A and B genes. However, after 72 h, chitin content was not significantly affected. Hence, validamycin can destroy the dynamic transformation balance of trehalose and glucose by inhibiting the activities of the two TREs, and further affect the expression of downstream chitin synthase genes. These findings provide a theoretical basis for using TRE inhibitors to control S. frugiperda.