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Carbendazim has the potential to induce oxidative stress, apoptosis, immunotoxicity and endocrine disruption during zebrafish larvae development

文献类型: 外文期刊

作者: Jiang, Jinhua 1 ; Wu, Shenggan 1 ; Wang, Yanhua 1 ; An, Xuehua 1 ; Cai, Leiming 1 ; Zhao, Xueping 1 ; Wu, Changxing 1 ;

作者机构: 1.Zhejiang Acad Agr Sci, Inst Qual & Stand Agroprod, State Key Lab Breeding Base Zhejiang Sustainable, Hangzhou 310021, Zhejiang, Peoples R China; Zhejiang Acad Agr Sci, Inst Qual & Stand Agroprod, Minist Agr, Key Lab Pesticide Residue Detect, Hangzhou 310021, Zhejiang, Peoples R China

关键词: Carbendazim;Zebrafish larvae;Oxidative stress;Apoptosis;Immunotoxicity;Endocrine disruption

期刊名称:TOXICOLOGY IN VITRO ( 影响因子:3.5; 五年影响因子:3.701 )

ISSN: 0887-2333

年卷期: 2015 年 29 卷 7 期

页码:

收录情况: SCI

摘要: Increasing evidence have suggested deleterious effects of carbendazim on reproduction, apoptosis, immunotoxicity and endocrine disruption in mice and rats, however, the developmental toxicity of carbendazim to aquatic organisms remains obscure. In the present study, we utilized zebrafish as an environmental monitoring model to characterize the effects of carbendazim on expression of genes related to oxidative stress, apoptosis, immunotoxicity and endocrine disruption during larval development. Different trends in gene expression were observed upon exposing the larvae to 4, 20, 100, and 500 mu g/L carbendazim for 4 and 8 d. The mRNA levels of catalase, glutathione peroxidase and manganese superoxide dismutase (CAT, GPX, and Mn/SOD) were up-regulated after exposure to different concentrations of carbendazim for 4 or 8 d. The up-regulation of p53, Apaf1, Cas8 and the down-regulation of Bcl2, Mdm2, Cas3 in the apoptosis pathway, as well as the increased expression of cytokines and chemokines, including OCCL-C1C, CCL1, IL-1b, IFN, IL-8, and TNF alpha, suggested carbendazim might trigger apoptosis and immune response during zebrafish larval development. In addition, the alteration of mRNA expression of VTG, ER alpha, ER beta 1, ER beta 2, TR alpha, TR beta, Dio1, and Dio2 indicated the potential of carbendazim to induce endocrine disruption in zebrafish lame. These data suggested that carbendazim could simultaneously induce multiple responses during zebrafish larval development, and bidirectional interactions among oxidative stress, apoptosis pathway, immune and endocrine systems might be present. (C) 2015 Elsevier Ltd. All rights reserved.

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